Ja’afar, Nur Sabrina (2021) Acute Toxicity Testing of Purple-fleshed Sweet Potato (Ipomoea batatas Var. Anggun) Leaf Extract Using Zebrafish (Danio rerio) Embryo Model. [Project Paper] (Submitted)
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Abstract
Ipomoea batatas (L.) Lam., (I. batatas) widely known as sweet potato, has been cultivated as a food crop for its tubers. Interestingly, previous experiments revealed that sweet potato leaves, a waste material from the cultivation of sweet potato, contain a high content of polyphenolics, namely anthocyanins and phenolic acids, compared with the major commercial vegetables. Despite its remarkable nutritional and medicinal value being increasingly recognized, published reports on the toxicity of sweet potato leaves are still limited. Objective: This study was designed to evaluate the acute toxicity effect of local purple-fleshed sweet potato (Ipomoea batatas var. Anggun) leaf extract using the zebrafish (Danio rerio) model. Methodology: Purple-fleshed I. batatas leaf extract was prepared using an ethanol (80% v/v) extraction method, followed by drying the extract using a freezer dryer. The acute toxicity effect of I. batatas leaf extract in different concentrations (0, 15.625, 31.25, 62.5, 125, 250, 500, and 1000 ug/mL) was evaluated using the zebrafish embryotoxicity test by observing the embryo's survival rate, hatching rate, and teratogenic changes for every 24 hours. Result: The median lethal concentration (LC50) value of I. batatas leaves ethanolic extract was 4130 ug/mL. There is no significant reduction (p-value > 0.05) in the percent of lethality, hatchability, heartbeat rate, and teratogenic alterations in the embryo treated with different concentrations of purple-fleshed I. batatas leaf ethanolic extract as compared with the corresponding control. Discussion: Based on the OECD Guideline no. 236, the LC50 value of purple-fleshed I. batatas leaf ethanolic extract using zebrafish embryo model was not included in any acute toxicity categories and considered safe for consumption. Conclusion: Findings from the study suggest that the exposure of D. rerio embryos to purple-fleshed I. batatas leaf ethanolic extract did not develop any significant toxic effects on D. rerio embryos. However, further toxicity assessment, particularly the chronic or genotoxic studies using repeated doses of I. batatas and other laboratory animals should be conducted to confirm its toxic effects.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 07:46 |
| Last Modified: | 22 Aug 2023 07:46 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1085 |
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