Assessment on wound closure of Gallic Acid-Loaded Graphene Oxide (GAGO) nano-formulation on 3T3 normal murine fibroblast

Zulkifle, Nur Aina Adlin (2021) Assessment on wound closure of Gallic Acid-Loaded Graphene Oxide (GAGO) nano-formulation on 3T3 normal murine fibroblast. [Project Paper] (Submitted)

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Abstract

A wound is defined as a damage to the skin or any tissue integrity interference with functional loss. It is due to the consequences of the epidermal layer integrity disruption. This is when the significance of wound healing process comes in actions. The wound healing mechanism begins instantly after the epidermal layer is damaged, and it may take days to years to heal. Inflammation, proliferation, and remodelling are the three stages of the wound healing system. Abnormal wound healing can occur if any of the wound healing phases are disrupted. Gallic acid (GA) is a hydroxybenzoic acid that commonly found in plants, and has been recognised for its antioxidant properties in wound healing. However, GA has a short half-life when administered in vivo. Nanotechnology advancement have facilitated researcher to overcome this issue. Graphene oxide (GO) has a unique structure and has been reported to be a potential nanocarrier for drugs. Objective: This study aims to assess the wound healing properties of newly formulated gallic acidloaded graphene oxide (GAGO) nanoformulation on 3T3-normal fibroblast cell line with comparison to its native compounds, pure GA and pure GO. Methodology: 3T3 fibroblast cell lines was treated with GAGO nanoformulation at eight different concentrations ranged between 5- 500uM. Scratch assay was performed to assess the wound closure up to 48 hr. Photo was taken daily and image analysis was done using ImageJ software. Results: In this study, the 3T3 cells that was treated with either pure GA, pure GO, or GAGO treatments showed a concentration-dependent manner. However, for 3T3-treated cells with pure GA and pure GO display a reduction in percentage of migration rate at high concentrations, while GAGO increases. At a high concentration of 500 uM, GAGO treatment increase the migration rate by more than 60% during the 12 hr post-treatment, and more than 80% during the 24 hr post-treatment. Discussion: The pure GA and GO contain its own properties in enhancing cells migration, but from 250 uM to 500 uM at 48 hr incubation period, there is a decrease in percentage of the migration rate of 3T3 cells due to the compound toxicity and inhibit the cells migration. The cells started shrinking and detaching from the surface at high concentrations causing a wide gap in the scratch area. Meanwhile, the combination of the compound into nanoformulation of GAGO, the 3T3-treated cells show a significance steady rise of the migration rate and lead to closure of the scratch area. Conclusion: The GAGO nanoparticles treatment accelerate the wound closure of 3T3 cells as compared to pure GA and GO within specific range of concentration.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:54
Last Modified:	22 Aug 2023 07:54
URI:	http://psaspb.upm.edu.my/id/eprint/1140

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