Determination of total phenolic content, total flavonoid content and antioxidant activities by cold aqueous, ethanol and methanol extractions of barnyard millet (echinochloa frumaentacea) and little millet (Panicum sumatrense)

Bostamam, Izzah Madihah Hannah (2020) Determination of total phenolic content, total flavonoid content and antioxidant activities by cold aqueous, ethanol and methanol extractions of barnyard millet (echinochloa frumaentacea) and little millet (Panicum sumatrense). [Project Paper]

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Abstract

Millet are small-seeded whole grains with various type and genetics of millet also known as drought-resistance crops with unique speciality that grown in tropical and semi-arid region which can withstand high temperature and dry condition. Millet are whole grains contain important source of fibre, vitamins, minerals, and phytochemicals. The aim of this study is to measure antioxidant contents and antioxidant activities of Barnyard millet and Little millet with three different extractions such as cold aqueous, 80% methanol and 70% ethanol. The antioxidant content and antioxidant activities were determined by using Total phenolic content (TPC), Total flavonoid content (TFC), Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging. One-way ANOVA and Pearson correlation was used in the study. Total phenolic content in Barnyard millet and Little millet are the highest in cold aqueous extract which are 1638.976mg GAE/g and 1318.633mg GAE/g compared with other extracts. Meanwhile for total flavonoid content, the results show 675.440mg CE/g and 825.627mg CE/g in cold aqueous extract of Barnyard millet and Little millet, respectively. The antioxidant activity using ferric reducing antioxidant power assay in cold aqueous were the highest value among other extracts in both Barnyard millet and Little millet which are 0.51mmol ferrous/g and 0.42mmol ferrous/g. For radical scavenging activity, the highest value is in 80% methanol extract for both Barnyard millet and Little millet which are 12.627% and 20.47% compared to the other extracts. There is a significantly positive correlations of phenolic content, flavonoid content with ferric reducing antioxidant power assay in both samples but there are no positive significant results in both samples using DPPH radical scavenging activity. Variations of millet used, solvent extract used and different concentration use may affect the study. Different extract in different sample can be additional information on antioxidant content and antioxidant activity in cereal grains. This information could potentially be exploited in the future to extract antioxidant sources especially from millet that can be applied in our daily diet.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	04 Sep 2023 07:19
Last Modified:	04 Sep 2023 07:19
URI:	http://psaspb.upm.edu.my/id/eprint/1266

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