Antimicrobial Susceptibility Patterns, Serotype Distribution, and Virulence Genes Among Invasive Streptococcus agalactiae Isolated from Malaysian Hospitals

Ali Al Salemi, Wardah (2020) Antimicrobial Susceptibility Patterns, Serotype Distribution, and Virulence Genes Among Invasive Streptococcus agalactiae Isolated from Malaysian Hospitals. [Project Paper] (Submitted)

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Abstract

Streptococcus agalactiae or group B streptococcus (GBS) is a normally harmless commensal bacterium which is part of the human microbiota that colonizes the body; however, GBS remains the leading cause of several infections in pregnant women, neonates and immunocompromised patients. In Malaysia, data on GBS infections concerning their serotype epidemiology, antimicrobial susceptibility, sequence types (STs) and virulence factors is very much lacking. Methodology: A total of 40 samples of invasive GBS were collected from five Malaysian hospitals starting from October 2019 to January 2020. The isolates were characterized by molecular serotyping and analysed for virulence genes content. Antimicrobial susceptibility was determined using disk diffusion, and the MICs for penicillin were determined by E-test. One selected invasive isolate that possessed multidrug resistance was analysed by multilocus sequence typing (MLST) to determine the genetic lineage. Results: Serotype V was the most common serotype (n = 12, 30%), followed by serotype Ia (n = 10, 25%), IV (n = 6, 15%), II (n = 5, 12.5%), III and VII (n = 2, 5% for each),and Ib, VI and IX (n = 1, 2.5% for each). All the isolates were sensitive to penicillin, vancomycin, ceftriaxone, cefotaxime, cefepime, ofloxacin, and linezolid, while 97.5%, 95%, 92.5, 92.5%, 85% and 12.5% of the isolates were sensitive to clindamycin, chloramphenicol, azithromycin, ampicillin, erythromycin, and tetracycline, respectively. PCR-virulence gene screening showed the presence of cfb, cylE, lmb, scpB, and hylB in all the isolates while bca and rib genes were found in 85 %, and 47.5 % of the isolates, respectively . The one isolate which was chosen for the sequence typing analysis using the multilocus sequence typing method belonged to ST 28. Conclusion: In conclusion, this study demonstrated that V and Ia are the predominant serotypes in this isolates collection and hence need to be considered while developing vaccines. The antibiotic susceptibility pattern showed local strains are susceptible to penicillin (the first- line antibiotic), indicating that it still could be used as the first choice for GBS treatment.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:08
Last Modified:	23 Aug 2023 00:08
URI:	http://psaspb.upm.edu.my/id/eprint/1284

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