Anti-proliferative activity of Acmella uliginosa flower extract on Human Colorectal Carcinoma cells (HCT-116)

Khor, Mei Hooi (2020) Anti-proliferative activity of Acmella uliginosa flower extract on Human Colorectal Carcinoma cells (HCT-116). [Project Paper] (Submitted)

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Abstract

Colorectal cancer (CRC) is rated as the third most common cancer among men and the second most common cancer among women. The survival rate of colorectal cancer has been greatly affected by metastasis as it may be fatal. In addition, the current cancer therapy treatments have high toxicities so alternative therapy with lesser adverse effects and greater effectiveness is urged to develop. Acmella uliginosa (AU), locally known as “Subang Nenek” or “Butang Baju Siti Fatimah” is a viable and flowering herb with various medicinal properties include antimicrobial, antioxidant, antinociceptive and anti-inflammatory properties. This study is conducted to determine the anti-proliferative activity of Acmella uliginosa flower extract (AUFE) on HCT 116 cells by assessing the cytotoxic effect and its anti-migratory potential towards HCT 116 cells. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) was conducted to evaluate the cell proliferation when treated with AUFE at the range of 0.5-2 mg/mL and inhibitory concentration (IC) observed at 50% of cell population (IC50) was determined with 24 hours incubation time. Next, wound scratch assay was followed to determine the anti-migratory potential of AUFE on HCT 116 cells which were tested at three different concentrations (0.21 mg/mL, 0.42 mg/mL and 0.63 mg/mL). 2 µg/mL of sunitinib was used as the positive control in wound scratch assay. From the MTT result, the IC50 obtained from HCT 116 cells when treated with AUFE was 1.125 ± 0.0052 mg/mL after 24 hours of incubation. AUFE treatment at concentrations ranging from 1.25 mg/mL to 2 mg/mL significantly reduced the HCT116 viability from 53.82 ± 4.45% to 15.6 ±6.43% when compared to untreated cells (p < 0.05). For wound scratch assay, the cells that have been treated with 0.42 mg/mL of AUFE showed the highest inhibition (78.89 ±5.75%) towards HCT116 migration. Whilst, AUFE at 0.63 mg/mL (68.82 ±9.88%) and 0.21mg/mL (25.97 ±14.01%) showed lesser cell migration when compare to untreated cells (0 mg/mL). The concentration of 0.63mg/mL and 0.42mg/mL had significantly inhibited the cell migration when compared to negative control (p<0.05), although the effect was not in a concentration dependent manner. From the MTT result, AUFE was able to inhibit 50% of cells proliferation when treated at the concentration of 1.125 mg/mL on HCT 116 cells which shows that AUFE exhibits anti-proliferative effect towards the colorectal cancer cell. The anti-migratory potential of AUFE also can be observed via wound scratch assay but there is no concentration dependent effect can be noticed in wound scratch assay. Treatment with AUFE can suppress the cell proliferation and inhibit the migration of HCT 116 cells in -vitro.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:32
Last Modified:	23 Aug 2023 00:32
URI:	http://psaspb.upm.edu.my/id/eprint/1291

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