Green fluorescent protein labelling of tumour targeting genetically modified Salmonella strains

Samynathan, Deepah (2020) Green fluorescent protein labelling of tumour targeting genetically modified Salmonella strains. [Project Paper] (Submitted)

Text FPSK2 2020 10.pdf Download (2MB)

Abstract

As the burden of cancer keep increasing, researchers focus on developing novel methods to treat and cure cancer. One of the novel anticancer studies is using genetically modified Salmonella as anticancer agents. Salmonella use for cancer treatments offers better solutions and effective approaches to eradicate the tumours. Before using Salmonella as a cancer treatment in clinical trials, it is crucial to understand the anticancer mechanism and cancer killing properties of the bacteria. To understand the interaction of bacteria with cancer cells in the complex tumour microenvironment, labelling and tracking of the bacteria is important. Green fluorescent protein (GFP) which emits bright green light on exposed to ultraviolet light will help to label and track the bacteria in tumour environment. Objectives: The objectives of this study are to label tumour targeting genetically modified Salmonella with GFP and to determine the effect of GFP plasmid on the growth of the transformed Salmonella. Hypothesis: It is hypothesised that the transformed Salmonella which carry the GFP plasmid do not show any changes in growth rate as compared to the untransformed Salmonella. Methodology: Three different Salmonella strains, S. Typhimurium, S. Agona and BDLA S. Agona were isolated from glycerol stock and underwent electrocompetency to be transformed into GFP-containing strains. The transformed strains were selected using ampicillin rich media and underwent fluorescence imaging and Bacterioscan analysis. Results: The transformed bacteria emitted bright green light when being observed under the fluorescence microscope. The Bacterioscan results showed that the GFP plasmid does not cause any prominent negative effect on bacterial growth. Conclusion: In summary, GFP is suitable to be used in labelling tumour targeting genetically modified Salmonella.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:56
Last Modified:	22 Aug 2023 07:56
URI:	http://psaspb.upm.edu.my/id/eprint/1292

Actions (login required)

View Item