The Effect of TGFβ1-induced Epithelial-Mesenhymal Transition on Autophagic Markers in Bronchial Epithelial Cells

Abd Manap, Amira Liyana (2020) The Effect of TGFβ1-induced Epithelial-Mesenhymal Transition on Autophagic Markers in Bronchial Epithelial Cells. [Project Paper] (Submitted)

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Abstract

Epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their epithelial phenotype and gain mesenchymal phenotype with enhanced migratory capacity and invasiveness, particularly in cancer cells. Besides cancer, EMT contributes to bronchial remodelling which is implicated in chronic inflammatory respiratory diseases such as asthma and chronic obstructive respiratory diseases (COPD). Transforming growth factor beta 1 (TGF-β1) is a potent inducer of EMT in both cancer and normal cells. TGF-β1 has also been shown to promote autophagy, at least in cancer cells. Autophagy is a catabolic intracellular pathway that degrades misfolded proteins and damaged organelles in order to maintain cellular homeostasis. Recently, autophagy has been implicated in asthma and COPD. However, the relationship between EMT and autophagy in the context of bronchial remodelling is presently unclear. Objective: Thus, this study aims to investigate the relationship between EMT and autophagy in human bronchial epithelial cells using TGFβ1 as an EMT inducer. The association between selected EMT (N-cadherin and Snail) and autophagic markers (Beclin-1 and LC3B) in TGF-β1-induced human bronchial epithelial cells was determined. Hypothesis: TGFβ1-induced EMT is associated with increased expression of the autophagic markers Beclin-1 and LC3B. Method: A normal human bronchial epithelial cell line, BEAS2B was cultured in LHC9 medium and induced with increasing concentrations (5, 10 and 20 ng/ml) of TGF-β1 for 48 hours. The protein expression of selected EMT and autophagic markers was then assessed by Western blotting. Data were analyzed by one-way ANOVA followed by Dunnet’s post hoc test. Result: The expression of EMT markers E-cadherin and Beclin-1 shows significant changes following induction by TGF-β1. The expression of autophagic markers Beclin-1 and LC3B shows no significant changes following TGFβ1-induced EMT in human bronchial epithelial cells. Conclusion: The results did not demonstrate a significant association between TGFβ1-induced EMT and autophagy in human bronchial epithelial cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:54
Last Modified:	22 Aug 2023 07:54
URI:	http://psaspb.upm.edu.my/id/eprint/1307

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