Screening of aqueous extracts of Bauhinia purpurea and Dicranopteris lineris leaves against human colon adenocarcinoma (HT-29) cancer cell line.

Mohd Yusoff, Amirah Alhusna (2020) Screening of aqueous extracts of Bauhinia purpurea and Dicranopteris lineris leaves against human colon adenocarcinoma (HT-29) cancer cell line. [Project Paper] (Submitted)

Text FPSK2 2020 21.pdf Download (2MB)

Abstract

Cancer is an abnormal growth of cells, of which many of the treatment options are associated with adverse side effects. Hence, there is a need to seek for bioactive compound(s) from natural sources, particularly plants, to develop a new drug that is safe with less or, possibly, no side effects for cancer treatment. Plant products (phytoextracts) are widely used in traditional medicine and are considered cheaper, safe, better alternative to synthetic drugs, without any side effects, and potential for disease targeting. In our attempt to contribute towards the development of new and alternative anticancer drugs to the currently available agents, the anticancer potential of two medicinal plants, namely Bauhinia purpurea L. (family Fabaceae) and Dicranopteris lineris L. (family Gleichiniaceae), which have been reported to possess remarkable antioxidant and anti-inflammatory effects, was studied. Objective: This study was done to investigate the cytotoxicity effect of aqueous extract of B. purpurea (AEBP) and D. linearis (AEDL) leaves against human colon adenocarcinoma cancer (HT-29). Methodology: In this study, cell viability was determined using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. HT-29 was plated at a density of 1 x 105. HT-29 was treated with a series of concentration (0.00, 7.81, 15.63, 31.25, 62.5, 125, 250, 500.00 and 1000 μg/ml) of AEBP and AEDL before incubated for 24, 48 and 72 hours. Result: The results show that AEBP exhibited the lowest percentage of cell viability at the concentration of 125 μg/ml (24 hours ≈ 68%), 250 μg/ml (48 hours ≈ 90%) and 1000 μg/ml (72 hours ≈ 87%). While AEDL shows the lowest percentage of cell viability at the concentration of 1000 μg/ml (24 hours ≈ 84%; 48 hours ≈ 90%; 72 hours ≈ 87%). Conclusion: Data obtained were not stable nd need to be replicated a few more times to obtain accurate results.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:48
Last Modified:	23 Aug 2023 00:48
URI:	http://psaspb.upm.edu.my/id/eprint/1309

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