Cytotoxic Properties of Novel Pyrazole Derivatives (TBBB47 and TBB47-A) on Human Breast Cancer (MDA-MB-231) and Normal Mouse Embryonic Fibroblast (NIH/3T3) Cells

Mohamad Arrif Emir, Widad Emir (2020) Cytotoxic Properties of Novel Pyrazole Derivatives (TBBB47 and TBB47-A) on Human Breast Cancer (MDA-MB-231) and Normal Mouse Embryonic Fibroblast (NIH/3T3) Cells. [Project Paper] (Submitted)

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Abstract

Metastasis, a significant characteristic of malignant tumours, is one of the most life-threatening pathological events in breast cancer. According to GLOBOCAN, breast cancer is the most prevailing type of malignant neoplasms among women with over one million new cases each year. Advanced chemotherapy developed therapy-resistant to major subpopulations in the world. Thus, further development for safer and more effective drugs against breast cancer is needed. In search of synthetic chemotherapeutic drug substances that can inhibit the process of multistage breast cancer, we have investigated the effects of two different novel pyrazole derivatives that potentially have the minimal killing of normal cells by using low dose concentration. The pyrazole urea (GeGe3) has been suggested to be a novel blocker of MAPK and P13K pathways and is implicated in inhibiting physiological and tumor angiogenesis. Herein, we aimed to continue from the previous study to evaluate the cytotoxic properties of TBBB47 and TBB47-A, two novel compounds of pyrazole derivatives from pyrazole urea (GeGe3). Methodology: Cell viability and cytotoxic effects of TBBB47 and TBB47-A on MDA-MB-231 and NIH/3T3 cells were measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. MDA-MB-231 and NIH/3T3 cells were grown in complete Dulbecco’s modified eagle medium and cultured under humidified conditions. Results: From MTT assay, TBBB47 and TBB47-A showed no toxic effect at concentrations of 1.56μM, 3.12μM, 6.25μM, 12.5μM, 25μM, 50μM and 100μM after three incubation periods on both cell lines. TBBB47 and TBB47-A showed cytotoxic effects at high concentration (>200μM) for both cell lines. To compare between two cell lines, both compounds showed similar pattern of cytotoxic effects on MDA-MB-231 and NIH/3T3 cells. Conclusion: TBBB47 and TBB47-A show cytotoxic effects on human breast cancer (MDA-MB-231) and normal mouse fibroblast cells (NIH/3T3). For the compounds to show cytotoxic effects on human breast cancer (MDA-MB-231) and normal mouse fibroblast cells (NIH/3T3), longer incubation period is required.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:39
Last Modified:	23 Aug 2023 00:39
URI:	http://psaspb.upm.edu.my/id/eprint/1312

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