Screening of Aqueous Extract of a Combination of Melastoma Malabathricum, Muntingia calabura, Bauhinia Purpurea and Dicranopteris Linearis Leaves Against HT-29

Mohd Zaini, Nur Hanis Suriani (2020) Screening of Aqueous Extract of a Combination of Melastoma Malabathricum, Muntingia calabura, Bauhinia Purpurea and Dicranopteris Linearis Leaves Against HT-29. [Project Paper] (Submitted)

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Abstract

Colorectal cancer is one of the most diagnosed and leading causes of cancer death worldwide. Despite the advancement in the field of drug-mediated cancer treatment, their effectiveness has been overshadowed by the development of various side effects. In an attempt to contribute towards the advancement of new anticancer drug discovery from natural products, this study evaluate the cytotoxic potential of several medicinal plants, namely a combination of aqueous extract of Melastoma malabathricum L. (family Melastomataceae) and Muntingia calabura L. (family Muntigiaceae), AEMMMC as well as the combination of aqueous extract of Bauhinia purpurea L. (family Fabaceae) and Dicranopteris linearis L. (family Gleicheniaceae), AEBPDL against human colorectal adenocarcinoma cell line (HT29). All these plants have been reported to exert various medicinal properties which are known to play a role in the mechanism of anticancer action. The leaves were mixed in a ratio of 1:1 for the combination of AEMMMC and AEBPDL and were extracted using ultrasonication with water as solvent and further freeze dried. Serial dilutions of different concentrations (7.8125 - 1000 µg/mL) were prepared to evaluate their anticancer activity using the in vitro MTT assay. The combination of AEMMMC demonstrated potential cytotoxic effect in reducing the cell viability of the HT-29 cancer cell line for 24, 48 and 72 hours post treatment. The cytotoxic effect of the extract increases in a dose-dependent manner which it shows a dramatic cytotoxic effect starting from concentration of 250µg/mL. However, the combination of AEBPDL showed no effect in reducing the cell viability of the HT-29 cancer cell line. In conclusion, the combination of AEMMMC has the potential as an anticancer agent against colorectal cancer. More comprehensive studies need to be done to evaluate its activity as an anticancer agent.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:49
Last Modified:	23 Aug 2023 00:49
URI:	http://psaspb.upm.edu.my/id/eprint/1313

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