ROS Status of Normal and Diabetic Murine Fibroblast-Treated with Gallic Acid Loaded Graphene Oxide (GAGO) Nano Formulation

Abdul Rahman, Nureen Alia (2020) ROS Status of Normal and Diabetic Murine Fibroblast-Treated with Gallic Acid Loaded Graphene Oxide (GAGO) Nano Formulation. [Project Paper] (Submitted)

Text FPSK2 2020 35.pdf Download (1MB)

Abstract

Diabetes Mellitus (DM), a state of chronic hyperglycaemia, affecting over 124 million individuals worldwide. One of the major complications of DM is impaired wound healing. Wound healing is a normal physiological process that proceeds through a series of co-ordinated cellular and cytokine-mediated events, culminating in the restoration of functional integrity of tissues. It has been reported that low levels of anti-oxidant accompanied by raised levels of markers of free radical damage may have contributory role in delaying the healing process in diabetic rats. Gallic acid (GA) is a phenolic compound found in almost all plants and has been reported to possess powerful health benefits such as anti-oxidant, anti-inflammatory, anti-cancer, and anti-diabetic properties. However, GA suffers a short half-life, in which affects its anti-oxidant property when administrated in vivo. Recent investigations have employed graphene oxide (GO), a biocompatible and cost-effective graphene derivative, as a nanocarrier for GA. There is a significant advantage of using nanomaterial in medicine for the diagnosis and treatment of diseases, especially in drug delivery. However, the effect of this formulated nano-compound has not been fully studied. Objective: Thus, this study aimed to evaluate the anti-oxidant level of gallic acid-loaded graphene oxide (GAGO) in normal and diabetic fibroblast. Hypothesis: It was hypothesized that better anti-oxidant level measured in 3T3 (normal fibroblast) and 3T3-L1 (diabetic fibroblast) treated with GAGO nano formulation compared to control groups. Methodology: Briefly, 3T3 normal fibroblast and 3T3-L1 diabetic fibroblast cell lines were treated with nine (9) different concentrations of GAGO nano formulation, ranged between 0-500 µM, for up to 24 hours. Reactive oxygen species (ROS) level was measured at 12 and 24 hours of post-treatment. As comparisons, pure GO and pure GA were used as controls in this study. Results: Our data showed a bell-shaped curve of ROS productions when 3T3 and 3T3-L1 cells were treated with pure GA, pure GO or GAGO nano formulation. All results showed time-dependent pattern. As compared to 12hr post-treatment, lower ROS productions were observed at 24hr post treatment of 3T3-treated with GAGO. Interestingly, the same observation was seen earlier in 3T3-L1 treated with GAGO, at 12hr post-treatment. Conclusion: Our results suggest that anti-oxidant effect of GAGO-treated cells is improved, both in 3T3-L1 and 3T3 cells. Altogether, this study indicates that GAGO nano formulation is worth to explore for its therapeutic properties. Therefore, further analysis is still required to establish the effects of ROS production and drug-release of this GAGO nano formulation, both in normal dan diabetic cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:54
Last Modified:	22 Aug 2023 07:54
URI:	http://psaspb.upm.edu.my/id/eprint/1325

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