Anuar, Nurul Syazrah (2020) Electron Microscopy Studies On The Effect Of Antimicrobial Peptides Derived From Pediococcus Acidilactici KP10 And Lactococcus Lactis GH1 On Vancomycin-Resistant Enterococcus And Methicillin-Resistant Staphylococcus Aureus. [Project Paper] (Submitted)
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Abstract
The emergence and dissemination of drug-resistant bacteria among major Gram-positive and Gram-negative species has exposed the public to severe health threats. As a result, the efficacy of conventional antibiotics has decreased dramatically over time and an imperative solution is urgently required to look for a more effective therapeutics agent against infections caused by antibiotic-resistant bacteria. Recent studies reported that antimicrobial peptides (AMPs) with potential novel mechanisms of inhibition exhibit significant potency against bacteria. The AMPs for this study are isolated from Pediococcus acidilactici KP10 and Lactococcus lactis gh1 and they are tested against vancomycin-resistant Enterococcus (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). Objective: This study generally aims to determine the effect of AMPs isolated from P.acidilactici KP10 and L.lactis gh1 on VRE and MRSA through microscopic analysis. Methodology: The Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the AMPs were determined against VRE and MRSA using Nutrient Broth (NB) as the growth medium. The bacteria cells were then treated with MIC values of respective AMPs or left untreated as control. Subsequently, the effect of the AMPs against the bacterial cells in NB was examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Result: Micrographs from SEM and TEM of MRSA and VRE bacterial cells treated with AMPs isolated from P.acidilactici KP10 and L.lactis gh1 displayed several distinguished and apparent signs of morphological and internal composition changes including rough surfaces, formation of blisters, pitted membranes and lysed cells. Conclusion: The growth of VRE and MRSA bacterial cells were inhibited by AMPs isolated from P.acidilactici KP10 and L.lactis gh1.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 23 Aug 2023 00:11 |
| Last Modified: | 23 Aug 2023 00:11 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1326 |
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