The Effect Of polyphenol-rich Extract Of Purple Sweet Potato (Ipomoea batatas) Leaves On Human Retinal Pigment Epithelial (ARPE-19) Cells Under High Glucose Condition

Hisamuddin, Ahmad Safiyyu’d-din (2020) The Effect Of polyphenol-rich Extract Of Purple Sweet Potato (Ipomoea batatas) Leaves On Human Retinal Pigment Epithelial (ARPE-19) Cells Under High Glucose Condition. [Project Paper] (Submitted)

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Abstract

Oxidative stress has been reported to play an essential role in the pathogenesis of diabetic retinopathy. Sweet potato (Ipomoea batatas) leaves, a necessary byproduct of sweet potato production, have been reported to have many beneficial pharmacological properties such as antioxidant, antiinflammatory, anticancer, antimicrobial and antiulcer activities. Objectives: This study is intended to evaluate the antioxidant and cytotoxic effect of polyphenol-rich extract of Ipomoea batatas leaves in human retinal pigment epithelial (ARPE-19) cells under high glucose condition. Methodology: The polyphenol-rich extract of purple sweet potato leaves (PSPL) was prepared by using 80% ethanol (w/v). The human retinal pigment epithelial (ARPE-19) cells were induced with high glucose concentration (30mmol/L) to mimic the diabetic retinopathy cell model. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine cell proliferation. The antioxidant properties of the extract were accessed by using the Ferric reducing antioxidant power (FRAP) assay. The morphology of the ARPE-19 cells was observed under the inverted light microscope. Result: The PSPL at a concentration of 7.8µg/mL to 500µg/mL exhibited no cytotoxicity effect. However, the PSPL at a concentration of 1000µg/mL demonstrated cytotoxicity effect towards ARPE-19 through morphological changes including cell shrinkage, cell floating and cell detachment. Besides, the 1000µg/mL PSPL showed significantly higher antioxidant activity as compared with control; vitamin C through the highest FRAP value [(1006.37±17.92)µM] and [(914.40±50.90)µM], respectively. Conclusion: Based potato leaves has a significant antioxidant activity with a low cytotoxic effect. These findings could be used as a foundation to develop therapeutic agents from PSPL to treat diabetic retinopathy and also to produce inexpensive natural antioxidant from local sweet potato leaves.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:12
Last Modified:	23 Aug 2023 00:12
URI:	http://psaspb.upm.edu.my/id/eprint/1328

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