Toxicity Assessment of Gallic Acid-Loaded Graphene Oxide (GAGO) Nano-Formulation on Diabetic Murine Fibroblast

Muner, Hani Nadiah (2020) Toxicity Assessment of Gallic Acid-Loaded Graphene Oxide (GAGO) Nano-Formulation on Diabetic Murine Fibroblast. [Project Paper] (Submitted)

Text FPSK2 2020 42.pdf Download (1MB)

Abstract

Diabetes Mellitus (DM) is a chronic disease associated with elevated blood sugar level due to impaired ability of the body to synthesis or respond to insulin. It is expected that 3.6 million Malaysians are suffering from DM and it is among the highest incidence in Asia and worldwide. One of the major complications of DM is impaired wound healing which leads to significant morbidity, specifically foot ulcer. Elevated blood sugar level results in delayed wound healing as the nutrient and oxygen is prevented from energizing the cells and affects the immune system. Gallic acid (GA) is a type of phenolic acid that is commonly found in plants, has been increasingly reported to have wound healing properties. However, GA suffers a short half-life when administered in vivo. In recent years, the advancement and development of nanotechnology have been used in various kinds of areas such as engineering, biotechnology, and also in medicine. Graphene oxide (GO) has a very unique structure and has been reported to be used as a drug carrier. Objective: This study aims to assess the toxicity of a new nano formulation known as gallic-acid loaded graphene oxide (GAGO), with comparison to its pure compounds, GA and GO on normal (3T3) and diabetic (3T3-L1) murine fibroblast cell line. Hypothesis: It is hypothesized that the toxicity of GAGO nano-formulation on 3T3- and 3T3-L1-treated cells is lower than those treated with pure GA or pure GO alone. Methodology: 8x104 of 3T3 and 3T3-L1 fibroblast cell lines were treated with GAGO nano-formulation at nine (9) different concentrations, ranged between 0-500µM. The cells viability was measured using MTT assay and data was recorded every 24hr for up to 72hr. As for comparison, pure GO and pure GA were used as controls in this study. Results: In this study, we observed that both 3T3 and 3T3-L1 cells showed time- and concentration-dependent patterns when they were treated with pure GA, pure GO or GAGO. Interestingly, while 3T3-treated cells show less than 20% of cell viability when treated with 500uM of GAGO, 60% of 3T3-L1-treated cells survived. In this study, 3T3-treated GAGO maintained high cell viability for at least 70% when treated with up to 250uM at 24-72hr post-treatment. In contrast, although 3T3-L1-treated GAGO show low cell viability at 24hr, cells number keeps increasing over time. This is explained by slower doubling time of 3T3-L1 compared to 3T3 cells. Conclusion: The cytotoxic effect of pure GA and pure GO on normal and diabetic murine fibroblast is reduced when GAGO nanoparticles treatment is given to the cells within a specific range of concentration.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:49
Last Modified:	23 Aug 2023 00:49
URI:	http://psaspb.upm.edu.my/id/eprint/1334

Actions (login required)

View Item