Assesment Of Ethanolic Extract Of Moringa Oleifera On The Viability Of Human Mesenchymal Stem Cells And Jurkat Leukaemic Cell Line

Mohamed Shtawi, Wafa Abdulhamed (2020) Assesment Of Ethanolic Extract Of Moringa Oleifera On The Viability Of Human Mesenchymal Stem Cells And Jurkat Leukaemic Cell Line. [Project Paper] (Submitted)

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Abstract

The use of mesenchymal stem cells (MSCs) to treat malignant diseases including leukaemia is gaining attention in the field of cell-based therapy, albeit, certain pre-clinical findings had described its tumour-promoting effects instead in the tumour microenvironment. Nonetheless, it has been unequivocally reported that MSCs may be primed with cytokines such as interferon- (IFN- ) to significantly exert its anti-tumour effects. However, the use of recombinant technology to synthesize growth factors such as IFN-  for cellular treatment have been known to be costly and prone to xenogeneic contamination to cause unwanted side effects. An innovative alternative that is cost-effective with significantly reduced risk of side effects has therefore being investigated over the years. Moringa oleifera (M. Oleifera), is a medicinal plant with anti-tumour properties capable of exerting cytotoxic effects to malignant A549 and BV-173 cells, among others. Objectives: We seek to investigate the effects M. oleifera ethanolic extract (MOETE) on the cellular viabilities of both MSCs and Jurkat T-cell leukemic cell line, as well as the effects on cellular viability when MSCs are co-cultured with Jurkat cells. Hypothesis: MOETE will enhance the cellular viability of MSCs while exerting opposite effects on Jurkat cells. Methods: MSCs were harvested by explant method from umbilical cord tissue and were characterized by immunophenotyping method using a flow cytometer, while Jurkat cells were purchased directly from the ATCC. MOETE was kindly donated by PhD student Mr. Ramesh Rangasammy Briefly, MSCs and Jurkat cells were treated with varying concentrations of MOETE, in which their percentage of cellular viabilities were then assessed via an MTT assay. A similar assay was also used to determine the percentage of cellular viabilities of Jurkat cells following its co-culture with varying MSCs densities. To further validate the results, Annexin V-FITC staining and evaluation was conducted to assess the apoptotic rate of MOETE-treated MSCs. Results: Harvested MSCs were determined positive for CD44, CD90, CD73, and CD105, while were negative for hematopoietic markers CD34, CD45, and immunogenic markers HLA-II, CD80, and CD86. MOETE increases the cellular viability of MSCs while had inhibited the proliferation of Jurkat cells, supported by the apoptotic rate findings. Interestingly, it has also been shown that the inhibition of Jurkat cells is also directly proportional to he densities of co-cultured MSCs. Conclusions: MOETE may significantly promote the cellular viability of MSCs. Also, both MOETE and MSCs had proved capable of inhibiting the proliferation of Jurkat cells.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	23 Aug 2023 00:47
Last Modified:	23 Aug 2023 00:47
URI:	http://psaspb.upm.edu.my/id/eprint/1340

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