Bamboo Charcoal (BC) From Gigantochloa albociliata as Potential Antibacterial Agent

Ahmad Khairi, Ahmad Khairul Aizad (2022) Bamboo Charcoal (BC) From Gigantochloa albociliata as Potential Antibacterial Agent. [Project Paper] (Submitted)

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Abstract

The rise of antibiotic-resistant bacteria left physicians with lessened weapons to combat these highly resistant pathogens and calls for the need to find new, effective, and possibly natural alternatives that possess an almost similar or better antibacterial activity to replace and reserve antibiotics as the final option against bacterial infections. Bamboo charcoal (BC) is theorized to possess potential antibacterial activity due to its high adsorptive property to trap and ideally halt the growth of bacteria. Despite the hype, the literature on the antibacterial potential of bamboo charcoal has shown to be inconsistent and requires the need for better designs with specifications on the genus and species of bamboo plant that is used to produce the BC. Gigantochloa albociliata, or also known as Malaysia buluh madu, is one of the native bamboo species that are found around Malaysia. Previous studies have demonstrated that the charcoal derived from this species has shown high adsorptive properties, which makes it a suitable candidate to test for the presence of potential antibacterial activity. Objective: This study intends to look at the potential antibacterial activity of bamboo charcoal (BC) derived from G. albociliata bamboo against selected bacteria. Hypothesis: It is postulated that the BC from G. albociliata can inhibit the growth of Gram-positive Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Susceptible Staphylococcus aureus (MSSA), and Gram-negative Escherichia coli and Klebsiella Pneumoniae. Methodology: MRSA, MSSA, E. coli and K. pneumoniae isolates were cultured together with BC in a broth dilution to observe the Minimum Inhibitory Concentration (MIC) of BC against the bacteria. Disc-diffusion tests were also performed to measure the diameter of the inhibition zone produced by the BC against bacteria. The concentrations of BC tested were fixed at three different concentrations: 1%, 5% and 10% (w/v) of BC. Results: Contrary to the hypothesis, none of the concentrations of BC tested showed any significant antibacterial activity. Discussion: Despite the existing literature supporting the high adsorptive property of the BC and the antibacterial activity of tested BC from other species of bamboo, it seems to be insufficient to produce any antibacterial effect as it may differ in the sense of pore size, genetic and chemical composition variability, and extraneous modifications. Conclusion: Bamboo charcoal (BC) that is derived from Gigantochloa albociliata bamboo does not possess antibacterial activity against MRSA, MSSA, E. coli and K. pneumoniae.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 04:36
Last Modified:	22 Aug 2023 04:36
URI:	http://psaspb.upm.edu.my/id/eprint/1355

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