Ng, Sing Jie (2022) Wound Healing Property of Gallic Acid-Loaded Graphene Oxide (GAGO) Nano-Formulation on Diabetic Murine Fibroblast. [Project Paper] (Submitted)
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Abstract
Diabetes mellitus (DM) is a chronic disease caused by insufficient insulin produced by the pancreas or ineffective use of insulin in body, which could cause delayed in wound healing. Wound happens when the integrity of the body tissues such as skin is damaged. Fibroblasts in skin dermal layer is vital to produce the connective tissue needed for wound healing. Gallic acid (GA) is a phenolic acid, which exists in most plants and it has been shown to have antioxidant features which enhance wound healing. However, when administered in vivo, GA has a short half-life. Nanomaterials are small in size, which is helpful to study the drug mechanisms and improve therapeutic effect. Graphene oxide (GO) is made up of a single-atom-thick layer of graphene sheets. GO properties including its physical (light), strength, thermal, and electrical conductivity make them ideal for active drug delivery. Objective: This study aims to assess the wound healing property of newly formulated Gallic Acid-loaded Graphene Oxide (GAGO) on diabetic murine fibroblast, with comparison to its native compounds, pure GA and pure GO. Methodology: Scratch assay was performed to assess the progress of wound closure on 3T3-L1 diabetic murine fibroblast-treated with GAGO nano-formulation at eight different concentrations (0μM-250μM). Photos were taken daily up to 72hr from each group and analysed using ImageJ software. Results: All treatment groups demonstrate concentration-dependent effects. Complete wound closure was observed in all treatment groups when treated with concentration <100μM. Although pure GA shows better wound closure at concentrations 100-200μM, it is toxic at 250μM to the cells. Interestingly, unlike pure GA and pure GO, GAGO significantly maintained its wound healing property up to 250μM. Discussion: Detachment of 3T3-L1 cells-treated with pure GA and pure GO, but not GAGO, observed at higher concentrations might be due to the toxicity effect of the pure compounds. Conclusion: These findings suggest that compared with pure GA and pure GO, encapsulation of GA on GO nano-carrier reduces the toxicity of this new formulation in diabetic fibroblast-treated cells. GAGO nano-formulation is comparable to pure GA while it improves wound healing performance when compared to pure GO.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 06:52 |
| Last Modified: | 22 Aug 2023 06:52 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1356 |
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