Mohd Faris, Nur Aina Nabilah (2022) Association of rs34911341 and rs696217 Polymorphism with The Risk of T2DM among Three Major Ethnics in Malaysia. [Project Paper] (Submitted)
![[img]](http://psaspb.upm.edu.my/style/images/fileicons/text.png) |
Text
FPSK2 2022 14.pdf Download (1MB) |
Abstract
Type 2 diabetes mellitus (T2DM), the most common type of diabetes mellitus is rapidly increasing worldwide including Malaysia. T2DM is a metabolic disease caused by the interaction of genetic and environmental factors. Insulin resistance (IR) and β-cell dysfunction are the main pathophysiological factors that contribute to the development of T2DM. Ghrelin is a peptide encoded by GHRL, known to play a role in glucose metabolism and in β-cell function. Polymorphisms in GHRL might play a role in the pathogenesis of T2DM. Objective: Thus, this study aimed to investigate the polymorphism effect of rs34911341 and rs696217 on the T2DM samples as well as with their serum ghrelin levels among three major Malaysian ethnicities. Methodology: Both rs34911341 and rs696217 of 298 DNA samples (T2DM: 150, non-T2DM: 148) were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum ghrelin levels of 87 T2DM samples were measured using Enzyme-Linked Immunosorbent Assay (ELISA). Results: This study found rs34911341 is significantly different between T2DM and non-T2DM (p=0.004) and between ethnicities of overall population (p=0.035) as well as among the non-T2DM samples (p=0.01). However, frequency of rs696217 genotypes in T2DM patients were similar to non-T2DM group. Although both rs34911341 and rs696217, and ethnicities showed no effect on serum ghrelin levels, interaction effect of ethnicity and rs696217 on serum ghrelin level was still found significant (p=0.033), particularly in Indian group (p=0.011). Discussion: The existence of different genotypes in both rs34911341 and s696217 indicates a heterogeneity of the ghrelin gene at these regions in the samples. Presence of association between ethnicities and genotypes of rs34911341 among non-T2DM samples but lack of the mentioned association among T2DM samples suggests that the T2DM samples were more homogenous in terms of genetic and lifestyle compared to non-T2DM group. The current study discovered that a majority of the T2DM serum samples’ ghrelin levels were towards the lower concentrations than higher concentrations which was similar to previous claims. Conclusion: This study suggested that rs34911341 polymorphism may confer risk of Malaysian T2DM without showing ethnic-specific associations meanwhile serum ghrelin level only showed association in the interaction of rs696217 and ethnicity.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 06:35 |
| Last Modified: | 22 Aug 2023 06:35 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1358 |
Actions (login required)
 |
View Item |