Comparison study on histopathological changes of the gastrointestinal tract after treatment of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) between BALB/C and C57BLK/6 Mice

Saubari, Nur Hidayah (2022) Comparison study on histopathological changes of the gastrointestinal tract after treatment of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) between BALB/C and C57BLK/6 Mice. [Project Paper] (Submitted)

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Abstract

The neurotoxin 1-methyl,4-phenyl-1,2,3,6-tetrahydropiridine (MPTP) is commonly used in rodents to induce experimental parkinsonism. Among various administration protocols, it has been reported that continuous or chronic exposure to small amounts of MPTP better mimics cell pathology resembling Parkinson's disease (PD). PD can give effects both motor (tremor, rigidity, bradykinesia, and posture instability) and non-motor symptoms (depression, olfactory impairment, and sleep disturbance). Apart from it, PD is also often presented with various gastrointestinal symptoms. Administration of neurotoxin (MPTP) in this study to different strains of mice BALB/c and C57BLK/6) to create a Parkinson's disease model and have a better understanding of the effects of MPTP on the gastrointestinal tract. However, due to different susceptibility to the MPTP in both strains, the differences in histopathological changes of the gastrointestinal tract because of MPTP administration has not yet been compared. It is also still controversial and not clear since previous studies showed MPTP works well in C57BLK/6 but still there are finding showing it also can cause an effect on BALB/c. Objective: To compare the effects of MPTP on the gastrointestinal tract between two inbred-mice strains (BALB/c and C57BLK/6). Methodology: 32 adult males of BALB/c and C57BLK/6 at age of 12 weeks were randomly divided into four groups, with each group comprised of four mice. The four groups were administered with normal saline, 15mg/kg, 30mg/kg, and 60mg/kg of MPTP respectively. After five days of treatments, all mice were sacrificed. The stomach, ileum, and descending colon were collected and fixed in formalin, dehydrated, and embedded in paraffin wax prior to sectioning for histological examination. The samples were stained with hematoxylin-eosin (H&E) and examined. Each photomicrograph taken was examined and scored based on three different categories of necrosis. The score was analyzed by using SPSS software version 25.0. Results: Increasing doses of MPTP can cause enhancement of necrosis formation in the stomach, ileum, and colon of C57BLK/6 if compared with BALB/c. It is proven by three different categories of necrosis show increasing trends in graph means and histopathology examination of three different gastrointestinal organs are more severe with increasing doses of MPTP in C57BLK/6 in comparison to BALB/c. Discussion: Our findings yielded MPTP also can cause effects of toxicity in both strains including BALB/c although it has the characteristics of resistance. However, it is only reasonable to see the effects of MPTP histologically in the gastrointestinal organs right after the administration of MPTP. The reason is that the BALB/c mice are expected to recover and reach control levels on a subsequent day, with a similar recovery observed in CBLK/6 on day 3. As the dose of MPTP is increased, the effect of MPTP toxicity is enhanced in C57BLK/6 compared to BALB/c. The possible mechanisms of differential sensitivity between those two strains are due to coat color, the different activity of MAO enzyme, a different expression of TH as well as a different expression of inflammatory mediators and oxidative markers. Conclusion: In short, the present study signifies that MPTP can cause effects of toxicity in both strains including BALB/c. Nonetheless, necrosis formation is enhanced with increasing doses of MPTP in C57BLK/6 compared to BALB/C.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:03
Last Modified:	22 Aug 2023 07:03
URI:	http://psaspb.upm.edu.my/id/eprint/1363

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