Molecular Characterization of Streptococcus pneumoniae Clinical Isolates at A Major Tertiary Hospital in Terengganu

Abdul Malik, Aiman Najihah (2022) Molecular Characterization of Streptococcus pneumoniae Clinical Isolates at A Major Tertiary Hospital in Terengganu. [Project Paper] (Submitted)

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Abstract

Introduction: Streptococcus pneumoniae is a Gram-positive diplococci bacteria that colonizes the upper respiratory tracts and causing various diseases such as pnuemonia, meningitis, otitis media and sepsi with incidence rate of 3.8 per 100,000 children in Malaysia. Trend of antibiotic resistant has become a concern globally and the spreading of globally disseminated lineage was predominance among the isolates. There have been studies to look at the prevalence and antimicrobial resistance pattern among pneumococci in Malaysia but geneotyping is still lacking that the genetic background of the circulating isolates in this country is largely unknown. Objective: This study aims to characterize and determine sequence types (ST) by Multilocus Sequence Typing (MLST) and phylogenetic analysis of the isolates from a major tertiary hospital in Terengganu. Methodology: A collection of pneumococcal isolates from a major tertiary hospital in Terengganu has been available from on-going studies and these isolates were re-identified by standard microbial identification for the confirmation of Streptococcus pneumoniae which were gram staining, bile test, catalase test and optochin susceptibility. Commercial extraction kit was utilized to conduct DNA extraction. Bacterial isolates were subjected to PCR and Multiplex PCR and were sequenced type by using MLST with the application of seven-housekeeping genes and phylogenetic analysis was performed to determine the relationship of the genetic lineage of the bacteria. Results: All of the STs shown are from globally and locally genetic lineage and no novel ST are observed in this study. Discussion: Based on phylogenetic tree, the isolates were branched into two clades, with one major clade consist of six STs (ST9, ST36, ST6195, ST3018, ST440 and ST180) and the other clade consist of two STs (ST12209 and ST81). All ST did not show MDR characteristics except ST81 which ST81 with serotype 6A observed in this study also has been observed in Korea which were related with Spain-23F clone. Conclusion: In conclusion, presence of globally and locally disseminated lineage with no novel ST in this study can help for better management of pneumococcal disease and policy.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 04:36
Last Modified:	22 Aug 2023 04:36
URI:	http://psaspb.upm.edu.my/id/eprint/1371

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