Mohd Noor Yusmadi, Nur Anina Nazirah (2022) Histopathological Examination on Pancreatic Tissues of Streptozotocin - Induced Diabetic Sprague Dawley Rats Treated with Purple Sweet Potato (Ipomoea batatas) Leaf Extract. [Project Paper] (Submitted)
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Abstract
Type 2 diabetes mellitus (T2DM) is a metabolic disorder that occurs due to the impairment of insulin to regulate the normal blood glucose level in the body. A few conventional medicines used in treating T2DM have been reported to develop unfavourable side effects on health. Hence, scientists keep investigating the potential of plant-based therapeutic agents to reduce the prevalence of T2DM among people. Purple sweet potato leaves have been identified as a functional food having a variety of bioactive chemicals shown to promote health. On the other hand, previously published studies have reported excessive oxidative stress in patients with T2DM can induce inflammation in the organs, resulting in premature cell senescence. Objective: The purpose of this study is to determine the effect of Ipomoea batatas leaf ethanolic extract (IBEE) on the diameter of Islets of Langerhans and the number of pancreatic β cells in treated rats. Methodology: A semi-quantitative histopathological assessment of the pancreas of treated rats was performed. The organs underwent a series of histopathological processes including tissue fixation, tissue processing, tissue embedding, tissue sectioning, slide staining with haematoxylin-eosin stain, microscopic examination and histopathological scoring. The data were analysed using one-way ANOVA, followed by Tukey’s post hoc test for multiple comparisons. Result: The results showed that oral administration of IBEE to streptozotocin-induced diabetic rats could improve the inflammation of the pancreas compared with those of untreated diabetic rats. Discussion: Plant ethanolic extracts have been suggested to contain an abundance of polyphenolic compounds which pharmacological effects include the anti-oxidation and anti-inflammation. Various phytochemical studies have reported that purple sweet potato leaf contain high content of polyphenolic compounds. The impressive protective effects of IBEE could be due to the phenolic and flavonoid compounds present in IBEE which possibly help scavenge the reactive oxygen species and regenerate the injured β cells. Conclusion: Findings from the study showed that IBEE has the potential to be developed as an alternative therapeutic agent to treat T2DM.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms. Nor Safa'aton Saidin |
| Date Deposited: | 22 Aug 2023 06:41 |
| Last Modified: | 22 Aug 2023 06:41 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1378 |
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