Antimicrobial Effects of Black Soldier Fly (BSF) extracts against pathogenic bacteria

Zamree, Lukhman Nul Ameen (2022) Antimicrobial Effects of Black Soldier Fly (BSF) extracts against pathogenic bacteria. [Project Paper] (Submitted)

Text FPSK2 2022 37.pdf Download (1MB)

Abstract

Most living creatures produce antimicrobial peptides (AMPs) as part of their immune system's defence against pathogenic microbes. AMPs are biological weapons that have evolved over millions of years, adapted to a harsh environment, and produce significant antimicrobial activity for the organism to survive. The widespread usage of antibiotics in recent decades has contributed to a false sense of security about the threat of bacterial resistance. AMPs are made up of distinct amino acid sequences and have different antibacterial properties that affect the growth of target organisms. Following adaptive immunity activation, significant types of AMP, such as defensins and cathelicidins, have been identified as playing a crucial role in innate immunity. Larvae of the black soldier fly (BSF) are well-known fly larvae that inhabit many countries around the world. BSF may have several AMPs to lessen the need for antibiotics to stop bacteria from spreading. Objective: This project aims to observe the activity of BSF extracts in inhibiting the growth of pathogenic bacteria through the measurement of inhibition zones. Methodology: MRSA, MSSA, E. coli, K. pneumoniae and S. typhi were isolated into a single colony and confirmed by using bacterial characterization tests. Different extracts, Hi. Dried, Hi. Protein and aqueous layers were carried out with varying concentrations of pure, 200 mg/mL, 100 mg/mL and 50 mg/mL. Disc diffusion method was used to determine the minimum inhibition concentration of MRSA, MSSA, E. coli, K. pneumoniae and S. typhi. Antibiotics were used as positive control while distilled water and DMSO were for negative control. Result: The inhibition zone of Hi. Protein extracts against bacteria were bigger compared to the inhibition zone of Hi. Dried extracts. Moreover, the pure extracts of Hi. Dried, Hi. Protein and aqueous layers showed higher antibacterial activity than the 200 mg/mL of extracts concentration. However, 100 mg/mL and 50 mg/mL of extracts concentration did not show any antibacterial activity against bacteria. Discussion: The initial weight of BSF larvae used for Hi.Protein and Hi.Dried was analogous. However, the drying process involved in Hi.Dried extracts resulted in the reduction of larvae mass. Hence, Hi.Protein extract was more concentrated than Hi.Dried extract. Pure extracts contain higher antimicrobial properties that interact with the tested bacteria Conclusion: Pure extracts showed higher antibacterial activity against MRSA, MSSA, E. coli, K. pneumoniae and S. typhi compared to other extracts.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 07:06
Last Modified:	22 Aug 2023 07:06
URI:	http://psaspb.upm.edu.my/id/eprint/1384

Actions (login required)

View Item