The Effects of Zerumbone on Hypoxia-Induced HCT116 Colon Cancer Cell Invasion

Aminnuddin, Fathin Dalila (2022) The Effects of Zerumbone on Hypoxia-Induced HCT116 Colon Cancer Cell Invasion. [Project Paper] (Submitted)

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Abstract

Colon cancer is the fourth leading cause of deaths by cancer throughout Asia by 2020. These deaths often occur in patients with metastatic stages, where their survival rate is greatly reduced compared to early-stage cancer patients. There had been a persistent increase in disease reoccurring despite having tumor resection, caused by the patients developing drug resistance against radiotherapy due to hypoxia. Zerumbone (ZER), a phytochemical is known for its antibacterial,antioxidant and anticancer properties. In addition, ZER is also known to possess therapeutic and chemopreventive properties in the treatment of cancer metastasis. Objective: Generally, this study is to determine the effects of ZER on the invasiveness of hypoxia-induced colon cancer cells (HCT116). Methodology: HCT116 cells were cultured and two assays were performed: MTT assay was performed to determine the cytotoxic effects of ZER towards HCT116 cells, while Transwell invasion assay was carried out to determine the effects of different concentration of ZER in the background of normoxic and hypoxic condition on the invasiveness of the HCT116 colon cancer cells. Results: IC20 and IC50 were determined using MTT assay. Under normoxic condition, IC20 and IC50 were 16 and 35 µM respectively, while under hypoxic condition, the values are 20 and 44 µM, respectively. Discussion: Based on the statistical analysis obtained, at 50 and 100 µM, there were significant decrease in cell viability in both normoxic and hypoxic background, both 24 and 48 hours of incubation. IC50 of ZER reduced the number of invasive cells and the concentration was significantly reduced to half its initial concentration (p<0.001) at 48 hours incubation, showing it is time-dependent Conclusion: ZER has cytotoxic and inhibitory effects on hypoxia-induced human colorectal carcinoma cell invasion, thus, reducing metastasis under hypoxic background.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms. Nor Safa'aton Saidin
Date Deposited:	22 Aug 2023 04:36
Last Modified:	22 Aug 2023 04:36
URI:	http://psaspb.upm.edu.my/id/eprint/1404

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