Determination of total lipid and omega 3 content in skin and flesh of chicken thigh from chicken fed with extruded flaxseed

Kamalrudin, Nabila Idzni (2020) Determination of total lipid and omega 3 content in skin and flesh of chicken thigh from chicken fed with extruded flaxseed. [Project Paper] (Submitted)

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Abstract

Among the terrestrial animals, chicken has the potential for increasing the n-3 PUFA intake in humans as they can synthesize eicosapentaenoic (EPA) and docosahexaenoic (DHA) from alpha-linolenic acid (ALA). Many researchers found that feeding chicken with food that rich in sources of n-3 PUFA such as flaxseed is effective in producing high n-3 PUFA chicken meat depends on the dietary treatment duration. Thus, this study aimed to determine and compare the total lipid, EPA and DHA content in skin and flesh of chicken thigh between chicken fed with common feed and feed enriched with extruded flaxseed on day 21st, 28th, 35th and 40th respectively. The total lipid was extracted using cold lipid extraction, while the EPA and DHA content were determined using acid- catalyzed fatty acid methylation method (FAME) and gas chromatography. The result showed that there was a significant difference (p<0.05) of total lipid and EPA content in the skin between the control and treatment group on day 28th, 35th and 40th. The EPA content in the skin was the highest and double up on day 40th. However, the total lipid content in the flesh remained the same after the dietary flaxseed treatment. There was also a significant difference (p<0.05) on day 28th and 40th for DHA content in the skin between the control and treatment group. However, the DHA content in both skin and flesh were decreasing in both control and treatment groups. In conclusion, the enrichment of chicken with high n-3 diet did not affect the total lipid content in the flesh. The longer the duration of flaxseed feeding, the more the increment of EPA can be seen in both skin and flesh. While for the DHA conversion was a tissue-specific type and this resulted in low DHA content in both skin and flesh.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	04 Sep 2023 03:40
Last Modified:	04 Sep 2023 03:40
URI:	http://psaspb.upm.edu.my/id/eprint/1438

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