Induced breeding and embryonic development of Systomus Rubripinnis (Valenciennes,1842)

James, Alvin (2023) Induced breeding and embryonic development of Systomus Rubripinnis (Valenciennes,1842). [Project Paper] (Submitted)

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Abstract

Introduction: This study was conducted to identify the different developmental phases of the embryo and larva, with a focus on the amount of time needed for each stage to develop as well as morphological differences between stages of this particular species. Problem statement: The most typical challenges in the aquaculture industry revolve around the production of offspring from boodstock. The complete embryonic development of S.rubripinnis has not yet be determined. Knowing the details on such information will aid in breeding this species in captivity thus, can be further commercialized for human consumption. It is critical to ensure that such species can maintain their population in order to avoid extinction, as well as to provide people with more information about the species in general. The efficient types of feed given need to be determined in order to produce healthy fish while keeping the budget as low as feasible in order to offer the best yields and maintain a financially sustainable culture system. Objective: The objective of this project is to conduct induced breeding using hormone and analyse and record the embryonic development of Systomus rubripinnis. Methodology: For the induced breeding of Sytomus rubripinnis, Salmon GnRH, Ovaprim ®Syndel containing 10 mg of domperidon and 20 µg of GnRH in per millilitre was injected as an inducing agent for ovulation of eggs inside the ovary. A Leica CME microscope with 40 to 100X inserts were used to take pictures of the eggs at intervals of one hour until hatching. Result and discussion: Well-fertilized eggs underwent significant morphological and physiological changes before developing into fry at various phases of embryonic development, from hatchlings to larvae to fry. The initial 3 stages from fertilized egg to blastula was completed within 21 hrs compared to following 3 stages from gastrula to hatchling which took 8 hrs. This shows that producing high-quality hatchlings rapidly is possible with proper hatchery management system. The yolk sac was absorbed within 72 hours during the larval stages, indicating the right time for the first external feeding. Conclusion: By using artificial fertilisation, larvae of the Javaen barb was succeeded to be obtained. Fertilised eggs are clear, demersal, spherical, and sticky eggs . The fertilised eggs had quite a mild brownish color. Information about the early life history, developmental stages, and start of the first feeding period for larval rearing were discovered through the current effort. Through an action research, the knowledge derived from this study might be recommended to hatchery operators. As a result, a hatchery might produce more eggs.

Item Type:	Project Paper
Faculty:	Faculty of Agriculture and Forestry Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	12 Oct 2023 07:19
Last Modified:	12 Oct 2023 07:19
URI:	http://psaspb.upm.edu.my/id/eprint/1441

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