Prevalence of viral nervous necrosis (VNN) and iridovirus infections in marine cage-cultured food fish at Pulau Ketam Selangor, Malaysia

Hussin, Nurus Sa'adatul A'abadiah (2020) Prevalence of viral nervous necrosis (VNN) and iridovirus infections in marine cage-cultured food fish at Pulau Ketam Selangor, Malaysia. [Project Paper] (Submitted)

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Abstract

Viral Nervous Necrosis (VNN) and Iridovirus (GIV) infections are common infections that can cause mass mortality and economic losses. The risk of the viral disease needs to be studied to prevent the losses. The objective of the current study was to determine the prevalence of Viral Nervous Necrosis (VNN) and Iridovirus (GIV) in a marine cage-cultured fish farm at Pulau Ketam, Selangor, Malaysia. A total of 15 grow-out fishes consisted of grouper (Epinephelus coides) (n=3), Red snapper (Latjanus campechanus) (n=6) and Asian seabass (Lates calcarifer) (n=6) were randomly sampled from the farm. The total body weight and body length of individual fish were measured and presence of clinical signs and abnormalities were noted. The brain and spleen were taken and fixed in 70% alcohol for virus detection using PCR, while brain, eyes, kidney and spleen samples were processed for histopathology. Clinical signs such as skin lesions i.e. loss of scale were observed in Asian seabass, while in grouper, skin lesion together with darkening of body. Laboratory results showed that the prevalence of VNN infection was 27% (4/15) while GIV infection was 0% (0/15) in total fish. The prevalence of VNN infection in grouper was the highest with 66% (2/3). As known, grouper could act as a natural reservoir of the virus. On other hands, 0% prevalence of Iridovirus infection in all three species could be due to the use of PCR kit that only detects Irido-Megalocytivirus, not (GIV). Histopathologically, vacuolative degeneration of the retina layer and in brain granular layer were observed in VNN+ve fish. This study found that VNN and GIV infection were at latent infection level in marine cage-cultured food fish at Pulau Ketam, Selangor, Malaysia.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	18 Oct 2023 07:09
Last Modified:	18 Oct 2023 07:09
URI:	http://psaspb.upm.edu.my/id/eprint/1451

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