Determination of the best immersion fixative for spleen, liver, and kidney tissues of the red tilapia fish (Oreochromis spp.)

Ismail, Abdul Aziz (2020) Determination of the best immersion fixative for spleen, liver, and kidney tissues of the red tilapia fish (Oreochromis spp.). [Project Paper] (Submitted)

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Abstract

Nowadays usage of fish in research has increased due to its importance in determining of toxic substances in the river environment as well as for diagnosing other fish diseases. Red tilapia fish (Oreochromis spp.) is one of the commonly used species in research and consumption in Malaysia. However, there is still a lack of information on the usage of suitable fixatives and its protocol on fish tissues especially spleen, liver and kidney. Thus, this study aims to determine the most optimum fixatives on kidney, spleen and liver tissues of red tilapia fish. A total number of 5 fishes obtained from a commercial farm in Beranang, Selangor were used in this study. Upon dissection, sets of organs consist of spleen, liver and kidney were harvested and immersed in four different types of fixatives which are Bouin’s fluid (B), Davidson’s fluid (D), 10% buffered formalin (BF1) and 20% buffered formalin (BF2). The samples were immersed in the respective fixatives for 24 hours and processed accordingly. Then staining was done using routine H&E stain and viewed under a microscope with an image analyzer. Five regions of interests per slide were randomly selected and scored on these parameters; overall morphological clarity of nuclei, staining intensity, the integrity of other tissues, lifting of the cartilage, space formation and the ease to section a specimen. The scoring data recorded were analyzed using the Kruskal Wallis test in SPSS. Our results showed BF1 and BF2 solutions are most suitable fixatives for the spleen. For liver, B recorded the highest total mean rank of score followed by BF1 and BF2 solutions. Meanwhile, D has the highest total mean rank of the score for the kidney followed by B and BF2 solution. In conclusion, different fixatives are suitable for different types of tissues. For further studies, it is suggested to consider other factors that may influences fixation of tissues such as the duration, mechanism on fixative penetration, pH, temperature and ratio of the sample size to fixatives volume during fixation of tissues.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	18 Oct 2023 07:12
Last Modified:	18 Oct 2023 07:12
URI:	http://psaspb.upm.edu.my/id/eprint/1452

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