Evaluation of micromorphological changes in lateral and medial condyle of the femur and tibia in both surgically and chemically induced osteoarthritic knee in a rabbit model

Yee, Xue Rou (2020) Evaluation of micromorphological changes in lateral and medial condyle of the femur and tibia in both surgically and chemically induced osteoarthritic knee in a rabbit model. [Project Paper] (Submitted)

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Abstract

Osteoarthritis is caused by inflammation, breakdown, and eventual loss of cartilage in the joints. Diagnosis of early stage of osteoarthritis is still under investigation due to the complicated pathogenesis of the disease. There are limited studies about the differences between the changes in medial and lateral condyle in knee joint during osteoarthritis thus this research was carried out. The objective of this study was to detect and evaluate the micromorphological changes in medial and lateral condyle in osteoarthritic femur and tibia such as bone to tissue volume (BV/TV), bone surface density (BS/TV), bone surface to volume ratio (BS/BV), trabecular thickness (Tb.Th) and trabecular seperation (Tb.Sp) in chemically-induced osteoarthritis and surgically-induced osteoarthritis at different time point (Week 4, 8 and 12). Thirty-five adult New Zealand white rabbits (n=35) were divided into three groups: Control (n=5), MIA (n=15) and ACLT (n=15). MIA and ACLT induced osteoarthritis were further divided into Week 4 (n=5), Week 8 (n=5) and Week 12 (n=5) groups. Right femur and tibia of each group of rabbit were harvested at different point of time and evaluated with micro-computed tomography (micro-CT) for the changes in the subchondral bone.. Data obtained from micro-CT were analysed statistically by performing factorial ANOVA for BV/TV, BS/TV and Tb.Sp followed by post-hoc Tukey test whereas Kruskal Wallis H test for Tb.Th. Results showed statistical significantly different (p<0.05) in BV/TV for medial and lateral condyles of femur and tibia at different point of time however the method of induction showed no statistical significantly different (p>0.05) in BV/TV. . The results indicate that the bone remodelling on both medial and lateral condyles were different from each other. Medial condyle showed more changes in subchondral bone due to imbalance bone reabsorption and formation process. Besides, there will be loss of subchondral bone in the early stages of OA but as disease progress there will be increase stiffening of sclerotic subchondral bone.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Oct 2023 03:19
Last Modified:	19 Oct 2023 03:19
URI:	http://psaspb.upm.edu.my/id/eprint/1470

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