Determination of common bacteria in Asian seabass Lates calcarifer (bloch, 1790) cultured in floating net-cages at Pulau Ketam, Selangor, Malaysia

Zulhisam, Atiqah (2020) Determination of common bacteria in Asian seabass Lates calcarifer (bloch, 1790) cultured in floating net-cages at Pulau Ketam, Selangor, Malaysia. [Project Paper] (Submitted)

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Abstract

Intensive aquaculture farming was one of the reasons for the rising number of disease outbreaks which could contribute to the decline in aquaculture production and increases the production cost. This research was carried out to determine the common species of the marine aquatic bacteria in Asian seabass reared in floating net-cages located at Pulau Ketam, Selangor, Malaysia. Samples of liver, kidney, intestines and from abnormal lesions were taken from ten Asian seabass. The inoculum from each sample was then streaked on the thiosulfate-citrate-bile salts-sucrose agar (TCBS) and marine trypticase soy agar (TSA added with 1% sodium chloride) and incubated at 25-30°C for 24 hours, aerobically. Water quality parameters were taken in the cages at the forward and the astern side of the farm, according to the water flow. Pure colonies obtained were stained with Gram’s stain and based on the microscopic examination, the cell morphologies were described. Identification of bacteria was conducted by using several biochemical tests such as oxidase, catalase, triple sugar iron, motility test, and API 20E test kit was used to confirm the bacteria genus and species. Then, the quantification of the bacterial loads was done with total plate count by using the spread plate method where a series of dilutions were plated on marine TSA. The results showed that Vibrio alginolyticus has the highest prevalence of 41.38%, while Stenotrophomonas maltophilia was 34.48%, followed by 20.69% of Photobacterium damselae and 6.9% of Shewanella putrefaciens. The total colony-forming unit (CFU) calculated from the Asian seabass kidney ranged from 2.9 x 103/ml to 1.9 x 104/ml in 2-5 dilution, in liver 7.4 x 103/ml in 2-4 dilution and in intestines 4.7 x 104/ml in 2-5 dilution. Based on the result findings, various recommendations on how to manage infectious disease outbreaks by pathogenic and opportunistic bacteria were suggested.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	19 Oct 2023 03:51
Last Modified:	19 Oct 2023 03:51
URI:	http://psaspb.upm.edu.my/id/eprint/1473

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