Molecular serotyping of streptococcus suis in porcine and feline

Tee, Jia Hui (2020) Molecular serotyping of streptococcus suis in porcine and feline. [Project Paper] (Submitted)

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Abstract

Streptococcus suis is a Gram-positive bacteria under Streptococcaceae family that acts as natural inhabitants of the porcine upper respiratory tract. It also cause inflammatory diseases in pig, mainly in weaned piglets, which characterized by septicaemia, meningitis and pneumonia. The isolation of S. suis from dogs, cats, ruminants, and horses has also been reported previously, indicating that S. suis has a broad host range. To control this problem in swine industry, serotyping of S. suis is crucial for effective local vaccine development and understanding of its pathogenesis. The objective of this study was to determine the serotypes of S. suis in the porcine and feline samples using multiplex Polymerase Chain Reaction (PCR) assay and serotype specific primer Polymerase Chain Reaction (PCR) assay. Four farms were selected from Selangor (2 farms), Penang and Perak (each 1 farm). The pigs selected included healthy and sick pigs suggestive of S suis. infection. For collection of sample in Selangor, fresh oral swabs have been obtained whereas in Penang and Perak, pigs were being euthanized and then lung tissue and pleural fluid were collected. On the other hand, a total of 30 porcine tonsil archived tissue samples and 30 lung archived tissue samples were retrieved. Furthermore, 40 cats’ archived serum samples which consist of 20 stray and 20 pet cats’ serum samples were retrieved as well. For fresh samples, DNA were extracted and subjected to S. suis detection PCR whereas for archived clinical samples that were in the form of extracted DNA, they were directly subjected to S. suis detection PCR. Furthermore, the S. suis-positive fresh samples then proceeded with multiplex PCR with focus on only Set 1 which include serotype 1 or 14, 2 or ½, 3, 7, 9, 11 and 16, whereas for the S. suis-positive archived samples, they were then proceeded with serotype specific primer PCR with focus on only serotype 2 and 9. In this study, Streptococcus suis was detected in 35 out of 70 (50%) porcine fresh and archived clinical samples collected, 1 out of 55 (1.81%) feline fresh and archived clinical samples, and lastly 0 out of 10 (0%) canine fresh clinical samples. Serotype 9 (19.44%) was the most prevalent serotype identified by this PCR assay, follow up by serotype 16 (2.22%) and serotype 2 (0.74%). On the other hand, 77.6% of the rest of the positive samples have to further carry out multiplex PCR for other serotypes under Set 2, 3 and 4 to detect other S. suis serotypes.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Oct 2023 06:50
Last Modified:	19 Oct 2023 06:50
URI:	http://psaspb.upm.edu.my/id/eprint/1485

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