Identification of ligand dependent nuclear receptor corepressor like (LCORL) gene polymorphism in sumbawa ponies (equus caballus) using PCR and DNA sequencing analysis method

Freddy, Freteldy (2020) Identification of ligand dependent nuclear receptor corepressor like (LCORL) gene polymorphism in sumbawa ponies (equus caballus) using PCR and DNA sequencing analysis method. [Project Paper] (Submitted)

Text lp FPV 2020 103.pdf Download (1MB)

Abstract

The Ligand Dependent Nuclear Receptor Corepressor Like (LCORL) is a protein coding gene suggested to have an association with the skeletal frame size and height measurements through its encoded transcription factor. Genome-wide association studies (GWAS) have discovered numerous polymorphisms within and outside of this gene involving its expression together with association towards physical traits such as body conformation across horse breeds. This study aims to identify the presence of LCORL gene polymorphisms in Sumbawa ponies (Equus caballus) using polymerase chain reaction (PCR) and DNA sequencing analysis method. A total of sixteen (n=16) blood samples were collected from healthy ponies with varying body composition. Samples were then subjected to DNA extraction and quality checked with a biophotometer. The extracted samples were then amplified via PCR method using primers designed from upstream of LCORL gene sequence. All samples showed the visualization of PCR bands with expected size between 300-400 base pairs (bp). After purification, sequencing and analysis the amplicons adjacent to LCORL was at 320 bp using the primer pair of study. Results showed the single nucleotide polymorphism (SNP) at the locus near LCORL which included T allele and C allele revealing twelve (12) ponies were homozygous TT and the other four (4) heterozygous TC. This SNP affects the transcription process of genes involved in the development of skeletal bone leading to an increased wither height. Overall, this study our findings demonstrated evident polymorphisms of the LCORL gene in these Sumbawa ponies and can be proposed as a candidate marker for strategic selection in breeding programs

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	23 Oct 2023 04:11
Last Modified:	23 Oct 2023 04:11
URI:	http://psaspb.upm.edu.my/id/eprint/1504

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