Daming, Roziana (2020) Molecular detection and genotyping of bartonella (proteobacteria: bartonellaceae) infecting peri-domestic rodents in Selangor, Malaysia. [Project Paper] (Submitted)
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Abstract
Bartonella are fastidous proteobacterium that are known to infect blood and endothelial cells, causing persistent bacteraemia in humans and a variety of animals. Several rodent-associated Bartonella species are human infective and are of public health concern. However, in spite of their zoonotic potential, information on the global distribution, hosts affinities, species diversity and disease epidemiology are limited. This study was therefore undertaken to determine the molecular prevalence, species identification and genetic diversity of Bartonella infecting peri-domestic rodents in Selangor, Malaysia. A total of 58 rodents were collected using small mammal mesh wire traps, from several residential and commercial areas in Selangor. The rodents were sedated with diethyl ether and anaesthetized with tiletamine/zolazepam. Blood was collected by cardiac puncture and stored and chilled in EDTA tubes. Following genomic DNA extraction, a partial fragment of the Bartonella 18SSUrRNA gene was amplified using genus-specific primers. Bartonella DNA was detected in 6.9% of the rats examined, and all positive rats were obtained from only one location. Infection was only present in the adult female rats at a prevalence of 11.1%. Infection rates in the two rodent species examined (Rattus norvegicus and R. rattus) were 6.5% and 7.4%, respectively. Bioinformatic and phylogenetic analyses of the 18SSUrRNA gene locus of four positive amplicons using maximum parsimony (MP) and neighbor-joining (NJ) algorithms revealed distinct clustering of the Malaysian isolates, with closest affinities to B. tribocorum, B. elizabethae, B. rattimassiliensis and Candidatus B. krasnovii. The presence of Bartonella among peri-domestic rodents in Selangor is a public health concern due to the potential for zoonotic transmission.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 23 Oct 2023 04:15 |
| Last Modified: | 23 Oct 2023 04:15 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1505 |
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