Potential of houseflies (Musca domestica) as a vector of Salmonella spp. and Escherichia coli in open house broiler farm in Johor, Malaysia

Mohd Nor, Ahmad Syamil (2020) Potential of houseflies (Musca domestica) as a vector of Salmonella spp. and Escherichia coli in open house broiler farm in Johor, Malaysia. [Project Paper] (Submitted)

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Abstract

Houseflies (Musca domestica) is one of many arthropods that coexist in broiler farms. Houseflies are attracted to decaying organic materials such as feces that are abundant in broiler farms. Feces contains a high number of microorganisms and pathogens. In particular, Salmonella spp. and Escherichia coli are the common microflora and can be transmitted to the broilers by the house flies. This study is to determine the presence of Salmonella spp. and Eschericia coli in house flies only in open broiler farms. A total of 30 pooled samples of houseflies was collected from 5 different broiler farms using a random sampling method. One pooled sample consists of 5 houseflies. External surface and internal content of the houseflies are the site of isolating Salmonella spp. and Escherichia coli. Salmonella spp. was cultured onto Xylose-Lysine-Deoxycholate Agar (XLD agar) and Brilliant-Green Agar (BGA). Escherichia coli was cultured onto Coliform-Chromogenic Agar (CCA). Biochemical test was done to confirm the identity of the colony. There was no Salmonella spp. isolated from both external surface and internal content of the 30 pooled samples. However, Escherichia coli was isolated in 21 out of 30 pooled samples (70%) in which 13 out of 30 pooled samples (43%) were isolated from the external surface and 14 out of 30 pooled samples (46%) were isolated from internal content. Hence, the possibilities of the houseflies to become the vector of transmitting Escherichia coli is high however the possibilities of the houseflies to become the vector of transmitting Salmonella spp. is low in this case.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	23 Oct 2023 07:15
Last Modified:	23 Oct 2023 07:15
URI:	http://psaspb.upm.edu.my/id/eprint/1512

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