Effect of systemic administration of granulocyte-colony stimulating factor on white blood cell count in rabbits

Mohd Robai, Zul Khidir (2020) Effect of systemic administration of granulocyte-colony stimulating factor on white blood cell count in rabbits. [Project Paper] (Submitted)

Text lp FPV 2020 66.pdf Download (1MB)

Abstract

Granulocyte-Colony Stimulating Factor (G-CSF) is a glycoprotein used to induce bone marrow production, proliferation of granulocytes and stem cells in human medicine. The potential use of G-CSF treatment in exotic animal practice is being evaluated to serve as an alternative treatment predominantly for any musculoskeletal injury to provoke production of inflammatory cells in the circulation for tissue repair and regeneration. Five (N=5) rabbits were used in this experimental clinical trial to determine the effect of G-CSF on the white blood cell count (WBC) and bone marrow response to the treatment given. The rabbits were subjected to subcutaneous injection of a placebo in the first phase and after a grace period of 14 days the same rabbits were subjected to subcutaneous injection of the G-CSF. The treatment regime was designed for 3 days, in which blood was collected daily for a span of 5 days, including day of pre-treatment and post treatments. Bone marrow samples were harvested for cytological study after euthanasia. Result on leukocyte count shows significant increase in the WBC components, primarily heterophils but declined about 48 hours after the last treatment. As for bone marrow cytology, notable elevated numbers of myeloid series cells and increment of the myeloid to erythroid (M:E) ratio observed. These findings prove that G-CSF is able to increase inflammatory cells and myeloid series cells in the bone marrow of rabbits. Thus, the potential future use of G-CSF for skeletal muscle injury in rabbits could be explored.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	23 Oct 2023 07:33
Last Modified:	23 Oct 2023 07:33
URI:	http://psaspb.upm.edu.my/id/eprint/1516

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