Comparison of pulmonary macrophage response between experimental buffalo with acute haemorrhagic septicaemia and carrier buffalo

Thanusha Raju, Thanusha Raju (2020) Comparison of pulmonary macrophage response between experimental buffalo with acute haemorrhagic septicaemia and carrier buffalo. [Project Paper] (Submitted)

Text lp FPV 2020 82.pdf Download (1MB)

Abstract

Pasteurella multocida is a normal flora of the upper respiratory tract of many animals including the domestic and wild bovids. P.multocida of specific serotype B:2 or E:2 are known to cause hemorrhagic septicaemia (HS). This disease is an acute and deadly form of pasteurellosis that affects mainly water buffalo, cattle, and bison. The first objective of this study was to compare the population of pulmonary macrophage between buffalo with acute and carrier HS. The second objective was to compare the phagocytosis rate of pulmonary macrophage between buffalo with acute and carrier HS. This study was done using histology slide samples from previous study where 6 buffaloes were used which were divided into 2 groups. Group 1 was infected with 1 ml of inoculum containing 105 cfu/ml of P. multocida B:2, intranasally (acute group). Group 2 were buffaloes which were not inoculated, but allowed to co-mingle with Group 1 buffaloes in order to create carrier animals. Buffaloes from Group 1 died within 68 to 72 hours post-inoculation and carrier animals were slaughtered. Samples of the lungs were previously fixed, processed, and stained with hematoxylin and eosin (H&E) and immunoperoxidase (IP) against whole cell P. multocida B:2. These slides were used in this study where the pulmonary macrophage response were compared with acute and carrier buffaloes. The slides stained with H&E were used to describe the lesions, and to score the population of pulmonary macrophages at 10 random high power fields (hpf). On the other hand, the slides stained with immunoperoxidase (IP) were used to score the population of macrophages with and without the positive immunostaining against P. multocida B:2 to determine the phagocytosis rate. Results showed that the acutely infected buffaloes had a higher macrophage population and phagocytosis rate compared to carrier buffaloes eventhough the significance of the data was not proven due to lack of data. The macrophage population and phagocytosis rate may play an important role in deciding the course of P.multocida B:2 infection in buffaloes.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	09 Nov 2023 03:33
Last Modified:	09 Nov 2023 03:33
URI:	http://psaspb.upm.edu.my/id/eprint/1523

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