Antimicrobial activity of propolis from Heterotrigona itama and Geniotrigona thoracica against Salmonella typhi and Escherichia coli

Bandi, Ummi Syamimi (2019) Antimicrobial activity of propolis from Heterotrigona itama and Geniotrigona thoracica against Salmonella typhi and Escherichia coli. [Project Paper] (Submitted)

Text lp FSPM 2019 22.pdf Download (4MB)

Abstract

Propolis is collected by worker honey bees from the trees. The bees pack propolis on their hind legs, carry it to their colony, and use it as a sterilant in the hive. Propolis has also been used by humans since early times for various purposes and, especially, as a meicine because of its antimicrobial properties. In this study, the ethanol extract of propolis produced by commonly found stingless bees, Heterolrigona itama inhibited the growth of Escherichia coli better than Geniotrigona thoracica but not excellently worked on inhibiting Salmonella typhi. The agar microdilution test shows the minimum antibiotic dilution needed to put E.coli bacteriostatic phase is on xio-10, H. itama on x I o-s, G. thoracica on x 1 o-6 while for S. typhi, the minimum antibiotic dilution needed is x I 0-2, and both H itama and G. thoracica are not effective on inhibiting S. typhi. Disc diffusion test shows significance difference on the mean of inhibition zone on antibiotic 24.60 + 1.30 and 24.60 ± 1.03 tested on E. coli and S. typhi respectively. Inhibition zone on both H. itama and G. thoracica has low significance difference. The result on H. itama are 6.00 ± 0.32 and 6.20 ± 0.20 respectively for S. typhi and E. coli, while on G. thoracica, 5.40 ± 0.24 and 6.20 ± 0.37 respectively for S. typhi and E. coli.

Item Type:	Project Paper
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	07 Feb 2024 07:23
Last Modified:	07 Feb 2024 07:23
URI:	http://psaspb.upm.edu.my/id/eprint/1625

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