The study on the effects of endosulfan on fertilization, hatching and survival rate of the lemon fin barb hybrid eggs and hatchlings

Mohd Nazri, Muhammad Nur Fikri (2022) The study on the effects of endosulfan on fertilization, hatching and survival rate of the lemon fin barb hybrid eggs and hatchlings. [Project Paper] (Submitted)

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Abstract

Extensive agriculture activities demand the use of pesticides. Pesticides could pollute the rivers and streams, possessed an environmental and health risk to aquatic life. Endosulfan (ES), an organochlorine insecticide, is still illegally used, despite the worldwide ban. In this study, the effect of ES on the fertilization, hatching and survival rate of the lemon fin barb hybrid (LFBH); (Hypsibarbus wetmorei × Barbonymus schwanenfeldii) eggs and hatchlings were examined. A pair of LFBH, was used as the broodstock. The sperm and eggs, harvested via the stripping method, were mixed and treated with different concentrations of ES; 0 ppm or control, 0.01 ppm, 0.1 ppm or 1 ppm. The fertilization and hatching rate were evaluated in vitro at 3 hr and 18 hr post-exposure, respectively (n=6/treatment). The survival rate of the larvae was assessed at 24 hr, 48 hr and 72 hr post-hatching (n=6/treatment). The LFBH eggs treated with 1 ppm ES demonstrated lower fertilization (44.24 ± 4.6%) and hatching rate (18.54 ± 2.8%) (p<0.05), than the control (63.35 ± 5.8% and 46.76 ± 1.3%, respectively). The main effect of treatment and time on the survival rate of the larvae was also significant (p<0.05; p=0.019 and p=0.09, respectively) within three days’ post-hatchlings. Overall, ES exposures display a detrimental effect on the early development and survival of the LFBH eggs and hatchlings. The findings in this preliminary study provide valuable insights for future studies on the toxicology of ES on other native freshwater fish species in Malaysia.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	13 Mar 2024 04:42
Last Modified:	07 May 2024 08:01
URI:	http://psaspb.upm.edu.my/id/eprint/1663

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