Effect of edible bird nest on testicular histomorphology, oxidative stress level and apoptotic biomarker of rats exposed to cadmium toxicity

Ganasan, Vaishnavi (2022) Effect of edible bird nest on testicular histomorphology, oxidative stress level and apoptotic biomarker of rats exposed to cadmium toxicity. [Project Paper] (Submitted)

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Abstract

Cadmium(Cd), a toxic heavy metal that is found in the environment as a pollutant, has the potential to cause damage to the male reproductive organs of mammals. Edible bird’s nest (EBN) produced from the swiftlet’s saliva has the properties of ameliorating the toxic effect of cadmium on testicles. Thirty Sprague Dawley rats aged 10 weeks old were used in the study to evaluate the protective effect of EBN on cadmium-induced testicular toxicity. Twenty-five rats randomly selected and grouped into 5: control group (given only Nacl 0.9%), and four other groups administered with 10mg/kg/day CdCl2 and four graded doses of 0, 60, 90 and 120mg/kg/day of EBN by oral gavage. After 16 days of treatment, the rats were sacrificed and testicles were collected. The left testis was used to study histomorphological changes and the right testis tissue homogenates were used to evaluate oxidative stress (superoxidase dimutase, malondialdehyde) and apoptotic biomarkers (caspase 3). Levels of superoxidase dimutase (SOD), malondialdehyde (MDA), and caspase 3 were measured using ELISA. Histomorphological studies showed significant differences in pathological changes in treated groups when compared with the control group. However, differences in the SOD, MDA, and caspase 3 levels were not significant. Histopathological scoring for groups treated with 0mg/kg EBN+10mg/kg CdCl2 ranked the least compared to 90mg/kg EBN treatment group suggesting a protective role of EBN against Cd toxicity effect on testicular tissues.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Mar 2024 07:04
Last Modified:	19 Mar 2024 07:04
URI:	http://psaspb.upm.edu.my/id/eprint/1673

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