Effects of edible bird’s nest on sperm quality and testosterone production in sprague dawley rats exposed to cadmium toxicity

Linus, Elisha (2022) Effects of edible bird’s nest on sperm quality and testosterone production in sprague dawley rats exposed to cadmium toxicity. [Project Paper] (Submitted)

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Abstract

Cadmium (Cd) is one of the major heavy metals exerting male reproductive toxicity, affecting endocrine function, spermatogenesis, semen quality and fertility. Edible Bird Nest (EBN) has proven to mitigate the toxic effects of Cd and lead acetate (LA) on the uterus of rats due to its positive proliferative and antioxidant properties. However, there is no study of EBN’s role in heavy metal toxicity in male rats. This study aimed to evaluate the EBN’s effect on sperm quality and testosterone production in rats. Twenty-five male Sprague-Dawley (SD) rats were randomly divided into five groups; Group 1 – control (NaCl 0.9%), Group 2 (CdCl2 10 mg/kg BW orally), while groups 3, 4, and 5 received CdCl2 (10 mg/kg BW) plus graded concentrations of 60, 90 and 120 mg/kg BW of EBN, respectively for 16 days. Rats were then euthanized, and testicles were weighed for organ coefficients. Cauda epididymis were collected for sperm quality evaluation (sperm concentration and sperm morphology), and blood samples were used for testosterone analysis using ELISA. Results showed no significant differences in organ coefficient between the groups. There was a significant drop in sperm concentration and testosterone, plus a significant elevation in the abnormal sperm rate in the Cd-treated group compared to the control and EBN-treated groups (p < 0.05). Compared to the Cd-treated group and EBN 60 mg/kg group, EBN 120 mg/kg group demonstrated a significantly higher sperm concentration and a lower percentage of abnormal sperm (p < 0.05). Likewise, the EBN 120 mg/kg group showed a significant increase in testosterone levels (p < 0.01) compared to the Cd-treated group. Therefore, EBN can avert the effect of Cd on sperm parameters and testosterone production at a dosage of 120 mg/kg.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Mar 2024 07:48
Last Modified:	19 Mar 2024 07:48
URI:	http://psaspb.upm.edu.my/id/eprint/1674

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