In vitro investigation on wound healing effects of porcine derived platelet lysate

Bustani, Shahrin Najmi (2022) In vitro investigation on wound healing effects of porcine derived platelet lysate. [Project Paper] (Submitted)

Text lp FPV 2022 19.pdf Download (1MB)

Abstract

Clinical application of platelet derivatives in wound healing is gaining popularity. By repeatedly freezing and thawing platelet-enriched blood samples, platelet lysate is created. It contains growth factors and cytokines that can promote tissue regeneration. Growth factors are crucial for the control of cellular processes involved in wound healing, including cell proliferation, angiogenesis, the creation and breakdown of extracellular matrix, and the migration of different cell types on the wound site. This study aimed to examine normal human dermal fibroblast response to porcine platelet lysate in terms of its ability to heal wounds. Therefore, to assess the impact of platelet lysate (PL) on improving wound closure under both typical physiologic and inflammatory circumstances, MTT assay and in vitro wound scratch tests of dermal fibroblast were performed. MTT assay measures cellular metabolic activity, it is an indicator for cell viability and proliferation. Different concentrations of platelet lysate (2.5%, 5%, 10% and 20% PRP-L and PPP-L), including positive and negative control (serum-free) were used in this experiment. According to the data, there is a significant difference between 20% PRPL and 2.5% PPPL. The effects of PL were then further examined with scratch wound closure assay to simulate cell spreading across a wound to form a new epithelial layer. Cells cultured in 24-well plates were mechanically scratched with a scratcher, and then monitored at 0, 24, 48 and 72 hours. Although statistical significance difference could not be shown, a general positive effect on scratch wound closure was observed with all PRP-L concentrations (5%, 10% and 20%) and 10% FBS (positive control) compared to PPP-L and serum-free medium (negative control). In conclusion, this study revealed that PL stimulates cell migration and proliferation, which promotes wound closure.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	19 Mar 2024 08:19
Last Modified:	19 Mar 2024 08:19
URI:	http://psaspb.upm.edu.my/id/eprint/1675

Actions (login required)

View Item