Evaluation of in vitro antibacterial and synergistic effect of garlic extract with enrofloxacin against Escherichia coli

Suresh, Ravenia (2022) Evaluation of in vitro antibacterial and synergistic effect of garlic extract with enrofloxacin against Escherichia coli. [Project Paper] (Submitted)

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Abstract

It is of utmost importance to search for new alternative sources of treatment quickly as the development of antimicrobial resistance is on the rise in the poultry industry. Garlic (Allium sativum) exhibits a wide range of antimicrobial properties and has been long used as traditional medicine in humans. However, the use of medicinal plants for the animal husbandry sector is rather scarce especially in Malaysia. This study aimed to investigate the antimicrobial activity and synergistic effect of garlic extract when used in combination with enrofloxacin against Escherichia coli. Ten fresh chicken fecal samples were obtained and plated on selective and differential media. Escherichia coli were identified by standard microbiological method (n=4). These four clinical isolates and one reference strain (ATCC 25922) of Escherichia Coli were further used in this study. The antibacterial activity of garlic extract and enrofloxacin were screened using disk diffusion method and their minimum inhibitory concentration (MIC) was determined by broth microdilution method. Combined efficacy of enrofloxacin and garlic extract was tested using disk diffusion method and was analyzed statistically. Results show that Escherichia coli was sensitive to garlic extract in all isolates. The MIC value of garlic extract in the clinical samples was in the range of 12.5%-25% while minimum bactericidal concentration (MBC) value for garlic extract was 25-50%. The synergistic effects of garlic extract were observed in the combination of enrofloxacin on the samples. The zone of inhibitions were significantly increased in combination of garlic extract with enrofloxacin. In conclusion, the garlic exhibits strong antibacterial properties against Escherichia coli and is highly efficient in enhancing the antibacterial activity of enrofloxacin.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	25 Mar 2024 03:05
Last Modified:	25 Mar 2024 03:05
URI:	http://psaspb.upm.edu.my/id/eprint/1678

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