Genetic variants and phylogenetic analysis of glasserella parasuis partial OmpP2 gene

Ong, Hui Xin (2022) Genetic variants and phylogenetic analysis of glasserella parasuis partial OmpP2 gene. [Project Paper] (Submitted)

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Abstract

Glaesserella parasuis or formerly known as Haemophilus parasuis, is a gram negative, pleomorphic, and microaerophilic bacterium under the Pasteurellaceae family. It is commonly known to cause Glässer’s disease, characterized by polyserositis, peritonitis, pleuritis, pericarditis, meningitis, and arthritis. Outer membrane protein P2 (OmpP2) is believed to be a virulence factor of Glaesserella parasuis. To further understand Glässer’s disease in local pig farms, molecular characterization and phylogenetic analysis of Glaesserella parasuis in OmpP2 gene is essential. Hence, the aim of this study is to detect OmpP2 gene in Glaesserella parasuis positive samples by using polymerase chain reaction (PCR) and run a hyplogenetic analysis between the local Glaesserella parasuis strains and international reference strain. A total of 38 Glaesserella parasuis positive archived samples from Selangor and Perak farm were used. DNA was extracted and subjected to conventional PCR assay for OmpP2 gene. The OmpP2 positive samples were purified and sent for gene sequencing. Phylogenetic analysis was performed on the local strain sequences in comparison to international reference sequences. OmpP2 gene was detected in 26 out of 38 samples (68.4%). Partial OmpP2 nucleotide comparison between 11 Malaysian isolates and 22 international reference strains showed that 10 out of 11 Malaysian isolates formed their own cluster in the phylogenetic tree, and were closely related to other strains, especially to China strains by sharing 90-98.7% homology. One OmpP2 isolate from Selangor farm showed high degree of homology to a virulent C5 strain from Hubei China (98.36%), SW114 strain from Hannover, Germany (98.37%) and D74 strain from Ames, USA (97.69%). In conclusion, this study provided new information regarding Glaesserella parasuis in Malaysia, in which this new insight might be useful for future related control studies.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	25 Mar 2024 03:20
Last Modified:	25 Mar 2024 03:20
URI:	http://psaspb.upm.edu.my/id/eprint/1701

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