Safety and immunogenicity of inactivated fowl adenovirus following different inactivation time intervals in broiler chickens

Mohammad Azreen, Adreen Qareena (2022) Safety and immunogenicity of inactivated fowl adenovirus following different inactivation time intervals in broiler chickens. [Project Paper] (Submitted)

Text lp FPV 2022 68.pdf Download (14MB)

Abstract

In recent years, much attention has been focused on the development of inactivated fowl adenovirus (FAdV) vaccines for the Malaysian poultry industry against inclusion body hepatitis (IBH) outbreak. It was the objective of this study to determine the safety and immunogenicity of inactivated FAdV following different inactivation time intervals in commercial broiler chickens. It was demonstrated that neither clinical signs, nor gross and histological lesions were recorded in all groups throughout the trial. The vaccine is safe without affecting the chicken performance throughout the trial since the body weight for all groups was no significant difference (p>0.05) at day 14 and day 28 post inoculation (pi). Based on ELISA findings, chickens vaccinated with inactivated FAdV at 32 hours (h) (group B) induce high antibody titer compared to that inactivated FAdV at 36h (group A) at day 14 and 28pi. At day 28pi, mean antibody titer for non-booster, groups A1 (321 ± 189) and B1 (602 ± 367) were significantly high (p<0.05) as compared to the control group (1± 0). Similarly, antibody titer for both booster groups, A2 (690 ± 484) and B2 (874 ± 317), are significantly high (p<0.05) compared to the control group (1 ± 0). Both FAdV vaccines using inactivated inoculum at 32h and 36h are safe and immunogenic in broiler chickens mainly at day 28pi following booster vaccination. It was concluded that the optimal period of FAdV inactivation was 32 hours and suitable to be used for future vaccine formulation.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	26 Mar 2024 03:44
Last Modified:	26 Mar 2024 03:44
URI:	http://psaspb.upm.edu.my/id/eprint/1705

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