Immunogenicity of inactivated fowl adenovirus vaccine candidate following different type of oil adjuvant in commercial broiler chicken

Mohamed Baijuri, Hanani Amni (2022) Immunogenicity of inactivated fowl adenovirus vaccine candidate following different type of oil adjuvant in commercial broiler chicken. [Project Paper] (Submitted)

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Abstract

In an effort to avert the rising cases of Inclusion Body hepatitis (IBH) due to Fowl Adenovirus serotype 8b in Malaysia, selecting a suitable adjuvant is vital in producing a vaccine with high immunogenicity against FAdV in commercial chicken. This study is aimed to determine the immunogenicity of inactivated FAdV vaccine candidates with different adjuvants. Forty-five (45) chickens were divided into groups A, B and C which were divided further into non-booster (groups A1 and B1) and booster (groups A2 and B2). Group A and B were inoculated with 0.2mL inactivated FAdV vaccine formulated with different adjuvants; Montanide ISA 71 VG and Montanide Gel P PR, respectively. Liver and trachea were collected together with serum sample and sent for ELISA for detection of FAdV antibody. Based on ELISA results, FAdV antibody (4469 ± 1175) were detected in day old chicks due to the presence of maternal antibody which significantly declined (p<0.05) at days 14 and 28 of age. Both vaccinated groups A and B induced antibody titer at day 14 post-inoculation (pi), however, there is no significant difference compared to control group due to neutralisation of antigen by maternal antibody. Interestingly, at day 28pi, antibody titer for booster group B2 (10871 ± 3871) were significantly high (p<0.05) as compared to group A2 (890 ± 645) and control group (1 ± 0). For non-booster group, mean antibody titer for group A1 were high (1237 ± 707) compared to B1 (950 ± 428) and control (1 ± 0) groups. There was no significant difference on body weight and antibody titer between booster and non-booster groups. It seems that inactivated FAdV vaccine formulated with Montanide Gel P PR adjuvant at 70:30 ratio is highly immunogenic in broiler chickens following booster at day 14pi. Thus, it is recommended for future inactivated FAdV vaccine against IBH in chickens.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	26 Mar 2024 03:46
Last Modified:	26 Mar 2024 03:46
URI:	http://psaspb.upm.edu.my/id/eprint/1706

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