Assessment of FXR and CYP7a1 gene expression in HepG2 cell lines supplemented with edible bird nest extract (EBNE) in modulating bile acid metabolism

Ibrahim, Nur’ain Suraya binti (2022) Assessment of FXR and CYP7a1 gene expression in HepG2 cell lines supplemented with edible bird nest extract (EBNE) in modulating bile acid metabolism. [Project Paper] (Submitted)

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Abstract

Edible bird’s nest (EBN) was derived from a salivary secretion of the male swiftlets. Aerodramus fuciphagus (white nests) and Aerodramus maximus (black nests) are two main species of swiftlets that are primarily recognized for their ability to produce high-quality bird nests. Despite giving many benefits towards human health, the effect of EBN on bile acid biosynthesis still remains elusive. The objective of this study is to determine the qualitative gene expression of FXR and CYP7al in HepG2 cell lines supplemented with EBN in the bile acid metabolism. The EBN was extracted and quantified prior to the cell treatment. The HepG2 cell was maintained in the Advanced Minimum Essential Medium (AMEM) and supplemented with 1% foetal bovine serum (FBS), 1% Penicillin-Streptomycin and Fungizone. The cells were supplemented with three different concentrations of the EBN extract (EBNE) which are 0.5, 1.0, and 1.5 mg/mL. Post 24 hours of supplementation, the RNA was extracted by using the RNeasy® Mini Kit, and conventional PCR was done to amplify the target RNA. Gel electrophoresis was conducted to visualise the band intensity of the genes. In this study, there is an upregulation of the CYP7a1 gene qualitatively in HepG2 cells supplemented with 0.5 and 1.0 mg/mL of EBNE concentration without affecting the FXR gene expression in HepG2 cell lines supplemented with the EBNE. The upregulation of CYP7a1 gene subsequently will modulate the down-stream bile acid metabolism in the liver cell and the supplementation would be beneficial in reducing the risk of cardiometabolic diseases.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 Apr 2024 04:56
Last Modified:	30 Apr 2024 04:56
URI:	http://psaspb.upm.edu.my/id/eprint/1726

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