Structural and optical properties of chitosan-1,8- naphthalimide thin film and its potential in glucose sensing using surface plasmon resonance

Ishak, Nur Izzati (2022) Structural and optical properties of chitosan-1,8- naphthalimide thin film and its potential in glucose sensing using surface plasmon resonance. [Project Paper] (Submitted)

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Abstract

In this study, the preparation of chitosan-1,8-naphthalimide (Chi/Napht) has been described. The obtained Chi/Napht thin film was then characterized by using Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) for structural properties and UV-Vis spectroscopy for optical properties. FTIR confirmed the functional group that excited in Chi/Napht such as carbonyl, carboxyl and hydroxyl group. Meanwhile, UV-Vis identified the absorbance value and optical band gap of Chi/Napht. The observed result was found between 220 nm to 300 nm for absorbance peak and 3.978 eV for optical band gap. Then the Chi/Napht thin film was incorporate with surface plasmon resonance (SPR) to identify its sensing potential towards glucose. The result showed positive response for different concentration of glucose and obtained that Chi/Napht thin film can detect glucose as low as 5 nM. The sensor produces a linear response for glucose with sensitivity of 1.0014° nM-1 for range 5 to 100 nM and 1.6578° x 10-4 nM-1 for 200 to 1000 nM of glucose concentration. Last but not least, AFM was used to identify the roughness of Chi/Napht thin film whereas the value was 0.179 µm before contact and 0.125 µm after the contact with glucose solution. Thus, this study is successful in determing the characteristic of Chi/Napht thin film and its potential in glucose sensing using SPR spectroscopy.

Item Type:	Project Paper
Faculty:	Fakulti Sains
Depositing User:	Ms Emelda Mohd Hamid
Date Deposited:	20 May 2024 02:24
Last Modified:	08 Aug 2024 07:58
URI:	http://psaspb.upm.edu.my/id/eprint/1750

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