Identification of canker disease of Khaya senegalensis in urban environment

Mohd Maznan, Mohamad Amirul Asyraf (2024) Identification of canker disease of Khaya senegalensis in urban environment. [Project Paper] (Submitted)

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Abstract

This study undertakes a comprehensive investigation into the canker disease affecting Khaya senegalensis, or Senegal Mahogany, within Malaysia's urban environment. Despite its resilience and widespread urban usage, this species faces recurring diseases that challenge its sustainability. The research has dual objectives: firstly, to characterize canker disease with lashing-like symptoms in Senegal Mahogany trees and, secondly, to identify the responsible pathogenic agent using a phylogenetic approach. Sampling locations were strategically chosen along Malaysian roadways, near the University Putra Malaysia (UPM) Serdang Campus, under the jurisdiction of Majlis Perbandaran Subang Jaya (MPSJ). Systematic sampling involved the collection of lashing canker disease specimens from infected Khaya senegalensis trees, ensuring a comprehensive representation from various wound conditions. Samples were isolated into Potato Dextrose Agar (PDA) media, with subsequent morphological identification under a Nikon Eclipse E200 compound microscope and molecular identification through Polymerase Chain Reaction (PCR) targeting the nuclear ribosomal DNA internal transcribed spacer (ITS) region. Phylogenetic analysis using MEGA11 software offered insights into evolutionary relationships among canker pathogenic agents. Out of 100 trees examined, three were confirmed to be infected with lashing canker disease, highlighting its subtle yet impactful nature. The identified pathogenic agent, tentatively classified as Lasiodiplodia spp., displayed distinct morphological characteristics such as the colony grows circularly with a soft texture and grows rapidly to the edge of the petri dish with greyish and fibrous mycelia which eventually turns black after 7 days of inoculation and with a black underside. Phylogenetic analysis indicates a close relation to Lasiodiplodia hormozganensis. This study underscores the importance of understanding and mitigating canker disease for the sustainability of Khaya senegalensis in urban landscapes.

Item Type:	Project Paper
Faculty:	Fakulti Perhutanan dan Alam Sekitar
Depositing User:	Ms. ROHANA ALIAS
Date Deposited:	14 May 2024 06:41
Last Modified:	01 Aug 2024 08:51
URI:	http://psaspb.upm.edu.my/id/eprint/1773

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