Detection of bunyaviridae virus rna among rattus species in Klang Valley by molecular method

Eleshamsor, Nur Aliah Batriesha (2022) Detection of bunyaviridae virus rna among rattus species in Klang Valley by molecular method. [Project Paper] (Submitted)

Text lp FPV 2022 77.pdf Download (1MB)

Abstract

In Asia and Europe, hantaviruses from the Bunyaviridae family are commonly associated with rodent-borne zoonotic viruses that can cause hemorrhagic fever with renal syndrome (HFRS) in humans. Some rat species act as a natural reservoir of hantaviruses that infect humans via inhalation of aerosolized urine, feces, saliva and bites. Previous serological study of hantavirus infection in rat samples was conducted in 2002 and has revealed that 2.52% of humans and 15.91% of rodents were positive of hantavirus in Port Klang and Kelantan, Malaysia. However, an updated study on the molecular prevalence of this virus in Malaysia is lacking. Therefore, this study was aimed to detect Bunyaviridae virus in the Rattus spp. in Klang Valley, Malaysia by molecular method. Blood serum and oropharyngeal swabs samples collected from 35 cage-trapped wild rats at three study sites within Klang Valley were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) targeting the S gene. The RT-PCR product was further analyzed by gel electrophoresis using 2% agarose gel and amplicon size of 930 bp was expected from the assay. One sample that showed a band approximate to the targeted amplicon size, regarded as a suspected positive sample, was sent for partial DNA sequencing. The RT -PCR and DNA sequencing analyses showed that Bunyaviridae RNA were absent from all 70 tested samples. This preliminary finding suggested that the rats from this study were not infected with Bunyaviridae related hantaviruses. However, a small sample size may obscure the true prevalence of the virus detection, therefore further study is needed to unravel the ambiguity.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	30 May 2024 07:40
Last Modified:	30 May 2024 07:40
URI:	http://psaspb.upm.edu.my/id/eprint/1841

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