Biogenic synthesis, characterisation and toxicity assessment of copper oxide nanoparticles (CUONPS) towards brine shrimp (ARTEMIA NAUPLII)

Rosli, Mhd Hazli (2022) Biogenic synthesis, characterisation and toxicity assessment of copper oxide nanoparticles (CUONPS) towards brine shrimp (ARTEMIA NAUPLII). [Project Paper] (Submitted)

Text FS 2022 31.pdf Download (3MB)

Abstract

The green approach is a rapidly growing area of nanotechnology research in which plant extracts were used to synthesise copper oxide nanoparticles (CuONPs). The plant extract such as Roselle (Hibiscus sabdariffa L.) were excellent as indigenous precursors of CuONPs. This study aims to synthesise CuONPs by using the sol-gel method and characterise the structural, chemical, optical, and thermal properties of CuONPs. Furthermore, this study explores the potential toxicity of CuONPs by using brine shrimp (Artemia Nauplii). Fourier-Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible Spectroscopy (UV-Vis), X-Ray Diffraction (XRD), Photoluminescence Spectroscopy (PL), Gas Chromatography-Mass Spectrometry (GC-MS), Dynamic Light Scattering (DLS), and Thermogravimetric analysis (TGA) were used to characterise the CuONPs. FTIR revealed the existence of numerous functional chemical groups of the CuONPs. The functional groups present on the surfaces of CuONPs consist of O-H, C-H, C=C, and C-O. XRD revealed a strong peak at 2θ = 36.59 o, with the diffraction of the (111) plane corresponding to CuONPs. According to XRD, CuONPs are monoclinic and crystalline. The UV-Vis absorption spectrum revealed the band stabilise ranging between 211 - 214 nm, attributed to the formation of CuONPs. PL spectra of CuONPs showed an excitation wavelength of 300 nm. The PL spectrum exhibits a UV emission peak at 308 nm, visible emission peaks in the blue region at 441 nm, and orange region at 618 nm. GC-MS demonstrated seven volatile compounds in the Roselle. Moreover, DLS exposes the average size distribution of CuONPs ranging from 168.4 - 291.4 nm. The average particle size and PDI revealed that the produced CuONPs are monodispersed. Similarly, the TGA curve showed the three-step decomposition of CuONPs. Lastly, brine shrimp were used to determine the nanotoxicity of CuONPs. CuONPs filter with 0.22 μm Polyethersulfone (PES) filter at concentration 0.0001 mg/mL showed the highest hatching percentage with 79.26 ± 9.52 %.

Item Type:	Project Paper
Faculty:	Fakulti Sains
Depositing User:	Ms. ROHANA ALIAS
Date Deposited:	20 May 2024 06:40
Last Modified:	05 Aug 2024 07:58
URI:	http://psaspb.upm.edu.my/id/eprint/1849

Actions (login required)

View Item