Ong, Kar Soon (2022) Occurrence of extended-spectrum beta-lactamase Escherichia coli at University Veterinary Hospital (UVH), Universiti Putra Malaysia. [Project Paper] (Submitted)
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Abstract
Antibiotics were one of the most useful drugs in veterinary and human medicine. However, antimicrobial resistance (AMR) had became one of the emerging public health concerns. Extended-spectrum beta-lactamase (ESBL)-producing E. coli is a widely distributed source of antimicrobial resistance for animals and humans. ESBL-producing E. coli has been reported in companion animals. The aim of this study was to detect and determine the occurrence of extended-spectrum beta-lactamase E. coli in warded animals, health premise environment, and personnel working at University Veterinary Hospital (UVH). Rectal swabs were collected from 8 warded animals and 7 resident animals, 30 staff, and 59 highly touchable surface in UVH. Swab samples were enriched in buffered peptone water and incubated overnight at 37oC. Enriched cultures were streaked on MacConkey agar supplemented with cefotaxime (1 ug/L) and incubated overnight at 37oC. Presumptive ESBL-E. coli were sub-cultured to obtain pure culture. A series of biochemical tests were conducted to confirm E. coli. The double disk diffusion (DDD) test was conducted to confirm the identification of ESBL-E. coli in a 2-step method, screening and confirmatory plates, based on the protocol by the Clinical and Laboratory Standard Institute (CLSI). A total of 20 presumptive ESBL-E. coli growth were obtained from the 104 samples, of which 11 were confirmed ESBL-E. coli. This data does not indicate significant wide-spread of resistance from pets to human or environment or inversely. This shows potential threat to public health, and highlights the need to foster IPC standards in veterinary health premises to prevent the spread of AMR into community.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 30 May 2024 08:35 |
| Last Modified: | 30 May 2024 08:35 |
| URI: | http://psaspb.upm.edu.my/id/eprint/1882 |
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